GAD-65 Antibody (A-3) recommended for detection of GAD-65 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

GAD-65 Antibody (A-3): sc-377145

GAD-65 Antibody (A-3) is rated 4.5 out of 5 by 6.
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Datasheets

    • Anti-GAD-65 Antibody (A-3) is a mouse monoclonal IgG1 (kappa light chain) GAD-65 antibody provided at 200 µg/ml
    • raised against amino acids 1-95 of GAD-65 of human origin
    • Anti-GAD-65 Antibody (A-3) is recommended for detection of GAD-65 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-GAD-65 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GAD-65 (A-3): sc-377145.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for GAD-65 Antibody (A-3) for WB and IHC(P) applications. This reagent is now offered in a bundle with GAD-65 Antibody (A-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Cat. SC-377145 comes conjugated with AF488?

Asked by: Patricia Guzman
Thank you for your question. GAD-65 Antibody (A-3): sc-377145 is available directly conjugated to Alexa Fluor® 488 (AF488). Please use the catalog number: sc-377145 AF488 to place your order for this conjugated version of the antibody.
Answered by: Technical Support
Date published: 2017-03-02

Can GAD-65 (A-3): sc-377145 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, GAD-65 (A-3): sc-377145 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from GAD-65 Confocal Immunocytochemistry of mouse primary cerebellar neurons conjugated with GAD-65 (1:200) and DAPI. Images collected by confocal microscope.
Date published: 2018-03-09
Rated 4 out of 5 by from Very good antibody for ICC I obtained some very nice images GAD-65 expression of my dissociated neuronal preps obtained from the striatum and cortex of E18.5 mouse brains.
Date published: 2017-09-18
Rated 5 out of 5 by from Great choice of antibody! Excellent antibody for IHC, strong and specific staining. In Western blot it gives solid specific banding at a 1:200 dilution.
Date published: 2017-01-19
Rated 4 out of 5 by from Strong Strong, crisp band seen in WB using IMR-32 whole cell lysate. -SCBT QC
Date published: 2015-06-16
Rated 4 out of 5 by from Positive result in WB using rat cerebellum Positive result in WB using rat cerebellum tissue extract and U-87 MG whole cell lysate. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-01-08
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