Anti-GAD-65 Antibody (A-3) is a mouse monoclonal IgG1 (kappa light chain) GAD-65 antibody provided at 200 µg/ml
raised against amino acids 1-95 of GAD-65 of human origin
Anti-GAD-65 Antibody (A-3) is recommended for detection of GAD-65 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-GAD-65 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GAD-65 (A-3): sc-377145.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for GAD-65 Antibody (A-3) for WB and IHC(P) applications. This reagent is now offered in a bundle with GAD-65 Antibody (A-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
GAD-65 Antibody (A-3) is a high quality monoclonal GAD-65 antibody (also designated GAD65 antibody, or GAD2 antibody) suitable for the detection of the GAD-65 protein of mouse, rat and human origin. GAD-65 Antibody (A-3) is available as both the non-conjugated anti-GAD-65 antibody form, as well as multiple conjugated forms of anti-GAD-65 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD-65 (also known as GAD2) and GAD-67 (also known as GAD1, GAD or SCP). GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67, the predominant GAD-67 form and the minor GAD-25 form. GAD-25 is not expressed in brain but can be found in a variety of endocrine tissues.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Thank you for your question. GAD-65 Antibody (A-3): sc-377145 is available directly conjugated to Alexa Fluor® 488 (AF488). Please use the catalog number: sc-377145 AF488 to place your order for this conjugated version of the antibody.
Answered by: Technical Support
Date published: 2017-03-02
Can GAD-65 (A-3): sc-377145 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?
Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, GAD-65 (A-3): sc-377145 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn