G3BP1 Antibody (H-10): sc-365338

G3BP1 Antibody (H-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects G3BP1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-G3BP1 antibody (H-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. G3BP1, also known as GTPase activating protein (SH3 domain) binding protein 1, G3BP, or HDH-VIII, plays a significant role in cellular processes. G3BP1 is predominantly localized in the cytoplasm of proliferating cells and translocates to the nucleus in non-proliferating cells, highlighting a dynamic nature in response to cellular states. G3BP1 functions as a sequence-specific, phosphorylation-dependent helicase, unwinding RNA and DNA duplexes, which is vital for processes such as DNA replication and RNA metabolism. G3BP1 acts as an endoribonuclease, cleaving mRNA at specific sites, thereby influencing gene expression and stability. G3BP1′s interaction with the SH3 domain of Ras GTPase-activating protein (Ras GAP) positions G3BP1 as a key regulator in the Ras signaling pathway, essential for cell growth and differentiation. G3BP1 expression is linked to the development of various cancers, including esophageal squamous carcinoma, making G3BP1 a significant target for research in cancer biology.

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G3BP1 Antibody (H-10) References:

1. Ras-GAP SH3 domain binding protein (G3BP) is a modulator of USP10, a novel human ubiquitin specific protease.  |  Soncini, C., et al. 2001. Oncogene. 20: 3869-79. PMID: 11439350
2. RasGAP-associated endoribonuclease G3Bp: selective RNA degradation and phosphorylation-dependent localization.  |  Tourrière, H., et al. 2001. Mol Cell Biol. 21: 7747-60. PMID: 11604510
3. The RasGAP-associated endoribonuclease G3BP assembles stress granules.  |  Tourrière, H., et al. 2003. J Cell Biol. 160: 823-31. PMID: 12642610
4. Rasputin, more promiscuous than ever: a review of G3BP.  |  Irvine, K., et al. 2004. Int J Dev Biol. 48: 1065-77. PMID: 15602692
5. Distinct structural features of caprin-1 mediate its interaction with G3BP-1 and its induction of phosphorylation of eukaryotic translation initiation factor 2alpha, entry to cytoplasmic stress granules, and selective interaction with a subset of mRNAs.  |  Solomon, S., et al. 2007. Mol Cell Biol. 27: 2324-42. PMID: 17210633
6. Modulation of p53 and MDM2 activity by novel interaction with Ras-GAP binding proteins (G3BP).  |  Kim, MM., et al. 2007. Oncogene. 26: 4209-15. PMID: 17297477
7. Expression of G3BP and RhoC in esophageal squamous carcinoma and their effect on prognosis.  |  Zhang, HZ., et al. 2007. World J Gastroenterol. 13: 4126-30. PMID: 17696235
8. The roles and mechanisms of G3BP1 in tumour promotion.  |  Zhang, CH., et al. 2019. J Drug Target. 27: 300-305. PMID: 30207743
9. Endometrial cancer (EC) derived G3BP1 overexpression and mutant promote EC tumorigenesis and metastasis via SPOP/ERα axis.  |  Ge, Y., et al. 2023. Cell Commun Signal. 21: 303. PMID: 37904149
10. DCAF7 Acts as A Scaffold to Recruit USP10 for G3BP1 Deubiquitylation and Facilitates Chemoresistance and Metastasis in Nasopharyngeal Carcinoma.  |  Li, QJ., et al. 2024. Adv Sci (Weinh). 11: e2403262. PMID: 38973296
11. A Ras-GTPase-activating protein SH3-domain-binding protein.  |  Parker, F., et al. 1996. Mol Cell Biol. 16: 2561-9. PMID: 8649363
12. Human DNA helicase VIII: a DNA and RNA helicase corresponding to the G3BP protein, an element of the ras transduction pathway.  |  Costa, M., et al. 1999. Nucleic Acids Res. 27: 817-21. PMID: 9889278

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-G3BP1 Antibody (H-10) has 77 citations in a variety of scientific publications.

Primary antibodies like Anti-G3BP1 Antibody (H-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

G3BP1 Antibody (H-10) Specifications

Alternate Product Name: G3BP1

Product Clone Name: H-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 158-251

Epitope Target: G3BP1

Epitope Species: human

Product Reactivity/Detection: G3BP1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 77 citations in various scientific publications

Available Conjugates/Forms for G3BP1 Antibody (H-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred G3BP1 antibody is G3BP1 Antibody (TT-Y): sc-81940.