Fra-1 Antibody (D-3) recommended for detection of Fra-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Fra-1 Antibody (D-3): sc-376148

Fra-1 Antibody (D-3) is rated 4.7 out of 5 by 6.
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Datasheets

    • Anti-Fra-1 Antibody (D-3) is a mouse monoclonal IgG1 (kappa light chain) Fra-1 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-29 at the N-terminus of Fra-1 of human origin
    • Anti-Fra-1 Antibody (D-3) is recommended for detection of Fra-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Fra-1 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-376148 X, 200 µg/0.1 ml)
    • blocking peptide, sc-376148 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Fra-1 (D-3): sc-376148.

Hello, I would like to use this antibody for ChIP. What dilution would you recommend? Do you have any validation data on the ChIP performance on this antibody?

Asked by: HesAmenya
Thank you for your question. This antibody has not yet been tested for ChIP, and we are not yet aware of any product citations using this particular antibody for any ChIP applications. We do extend our warranty to cover that research application for our TransCruz reagent for Gel Supershift and ChIP applications (sc-376148 X, 200 µg/0.1 ml). We currently offer a different antibody that has been published for use in ChIP applications. Please refer to our Fra-1 Antibody (C-12): sc-28310. And PMID's: 22198213 and 21088499.
Answered by: Technical Support
Date published: 2020-07-23

Welche Proteinmenge eignet sich am besten für einen Western Blot mit dem Fra-1 Antikörper?

Asked by: Neslihan
Vielen Dank für Ihre Frage. Die optimale Proteinmenge ist abhängig von dem verwendeten Sample. Generell empfehlen wir pro Bahn bei der Elektrophorese eine Proteinmenge von 40–60 µg bei Zelllysaten, 10–20 µg bei Kernextrakten, 5-10 µg bei transfizierten Zelllysaten, oder 10–20 ng bei aufgereinigtem Protein. What amount of protein is ideal for a western blot with Fra-1 Antibody (D-3): sc-376148? Thank you for your question. The ideal amount of protein is dependent on the sample that is being tested. We generally recommend 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

I would like to use Fra-1 Antibody (D-3): sc-376148 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-376148 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2017-01-31
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Rated 5 out of 5 by from good quality received the samples weeks ago. blotting showed very strong signal
Date published: 2018-02-19
Rated 3 out of 5 by from Average features work well in WB at 1:500,Ges-1 Ccell,but when I recycle to use it,the titer is too inefficient,which led to a poor result.
Date published: 2017-12-05
Rated 5 out of 5 by from Works well for western blot The antibody produced a good western blot for human tissue samples
Date published: 2016-12-19
Rated 5 out of 5 by from Positive detection of endogenously expressed Fra Positive detection of endogenously expressed Fra-1 protein in NIH/3T3 cells (nuclear extracts used) through analysis by Western Blot. -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Excellent immunoperoxidase nuclear and cytoplasmic Excellent immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-03-08
Rated 5 out of 5 by from Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Fra-1 protein via Western Blot analysis. -SCBT QC
Date published: 2015-02-04
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