Fra1 Antibody (C-12): sc-28310

Fra-1 Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 1-50 of human Fra-1, a crucial transcription factor in the Fos protein family. Anti-Fra-1 antibody (C-12) recognizes an epitope within this N-terminal region and demonstrates broad species cross-reactivity, detecting Fra-1 in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. As a nuclear phosphoprotein also known as Fos-related antigen 1, FRA1, or FOSL1, Fra-1 functions through a sophisticated mechanism involving its basic leucine zipper (bZIP) domain, which enables heterodimerization with Jun family proteins to form the activator protein-1 (AP-1) complex. This complex binds to specific DNA response elements, regulating genes involved in cell proliferation, differentiation, and survival. Fra-1′s activity undergoes precise control through post-translational modifications, particularly phosphorylation by kinases such as ERK, which stabilizes Fra-1 by preventing its degradation via the ubiquitin-proteasome pathway. This stabilization mechanism takes on particular significance in cancer biology, where Fra-1 overexpression and accumulation can drive tumor progression and metastasis. Through these diverse cellular functions and regulatory mechanisms, Fra-1 (C-12) antibody provides researchers with a valuable tool for investigating both normal physiology and disease pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Fra1 Antibody (C-12) References:

1. FRA-1 protein overexpression is a feature of hyperplastic and neoplastic breast disorders.  |  Chiappetta, G., et al. 2007. BMC Cancer. 7: 17. PMID: 17254320
2. Fra-1 promotes growth and survival in RAS-transformed thyroid cells by controlling cyclin A transcription.  |  Casalino, L., et al. 2007. EMBO J. 26: 1878-90. PMID: 17347653
3. Isolation and characterization of fra-2, an additional member of the fos gene family.  |  Nishina, H., et al. 1990. Proc Natl Acad Sci U S A. 87: 3619-23. PMID: 2110368
4. The product of a fos-related gene, fra-1, binds cooperatively to the AP-1 site with Jun: transcription factor AP-1 is comprised of multiple protein complexes.  |  Cohen, DR., et al. 1989. Genes Dev. 3: 173-84. PMID: 2497053
5. An avian transforming retrovirus isolated from a nephroblastoma that carries the fos gene as the oncogene.  |  Nishizawa, M., et al. 1987. J Virol. 61: 3733-40. PMID: 2824811
6. Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1.  |  Bohmann, D., et al. 1987. Science. 238: 1386-92. PMID: 2825349
7. FRA-1 suppresses apoptosis of Helicobacter pylori infected MGC-803 cells.  |  Yang, Y., et al. 2021. Mol Biol Rep. 48: 611-621. PMID: 33389529
8. The Fos protein complex is associated with DNA in isolated nuclei and binds to DNA cellulose.  |  Sambucetti, LC. and Curran, T. 1986. Science. 234: 1417-9. PMID: 3491427
9. FRA-1 as a Regulator of EMT and Metastasis in Breast Cancer.  |  Casalino, L., et al. 2023. Int J Mol Sci. 24: PMID: 37176013
10. Virus induction of osteosarcomas in mice.  |  Finkel, MP., et al. 1966. Science. 151: 698-701. PMID: 5218049
11. Regulation of Fra-1 and Fra-2 phosphorylation differs during the cell cycle of fibroblasts and phosphorylation in vitro by MAP kinase affects DNA binding activity.  |  Gruda, MC., et al. 1994. Oncogene. 9: 2537-47. PMID: 8058317
12. The NFAT-1 DNA binding complex in activated T cells contains Fra-1 and JunB.  |  Boise, LH., et al. 1993. Mol Cell Biol. 13: 1911-9. PMID: 8441422

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Fra1 Antibody (C-12) has 57 citations in a variety of scientific publications.

Primary antibodies like Anti-Fra1 Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Fra1 Antibody (C-12) Specifications

Alternate Product Name: Fra-1

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-50

Epitope Target: Fra-1

Epitope Species: human

Product Reactivity/Detection: Fra-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 57 citations in various scientific publications

Available Conjugates/Forms for Fra1 Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent