FOXM1 Antibody (G-5) recommended for detection of all isoforms of FOXM1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

FOXM1 Antibody (G-5): sc-376471

FOXM1 Antibody (G-5) is rated 4.0 out of 5 by 25.
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Datasheets

    • Anti-FOXM1 Antibody (G-5) is a mouse monoclonal IgG2b (kappa light chain) FOXM1 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 734-763 at the C-terminus of FOXM1 of human origin
    • Anti-FOXM1 Antibody (G-5) is recommended for detection of all isoforms of FOXM1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-FOXM1 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-376471 X, 200 µg/0.1 ml)
    • blocking peptide, sc-376471 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of FOXM1 (G-5): sc-376471.
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FOXM1 G-5 is mouse monoclonal IgG2b. I would like to know if it can be used with conventional secondary anti-mouse IgG reagents.

Asked by: Gemo
Thank you for your question. Yes, you can use a conventional anti-mouse IgG secondary antibody for detection of this primary antibody. For more specific results, we do recommend using an isotype-specific secondary antibody (anti-mouse IgG2b), or our mouse IgGκ binding protein, e.g., m-IgGκ BP-HRP: sc-516102.
Answered by: Technical Support
Date published: 2020-03-09

Cantidad de anticuerpo FOXM1(G5) X TransCruz sc-376471 recomendado para usar en ChIP.

Asked by: femili12
Gracias por sus preguntas. Recomendamos el uso de 100-500 μg de sobrenadante y 0,1-1 μl de reactivo TransCruz (0,2-2 μg). Para obtener más información, haga clic en este enlace de nuestro protocolo ChIP: https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays Thank you for your questions. We recommend using 100–500 µg supernatant and 0.1-1 µl TransCruz reagent (0.2–2 µg). For further information, please click on this link to our ChIP protocol: https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays
Answered by: Technical Support
Date published: 2018-12-31

Which type of FOXM1  give good results for IHC on parrafin bock,human tissue breast cancer

Asked by: Madyan
Thank you for your question. We would recommend using our mouse monoclonal antibody, FOXM1 (G-5): sc-376471. We have several pieces of IHC(P) data in human tissue using this antibody on our website.
Answered by: Technical Support
Date published: 2017-08-22

Does the FOXM1 Antibody (G-5): sc-376471 detect isoform “C” of the FOXM1 target?

Asked by: Trav11
Thank you for your question. The FOXM1 Antibody (G-5): sc-376471 will detect all isoforms (A, B, & C) for the FOXM1 protein target. If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 15
Date published: 2017-01-16

Does FOXM1 Antibody (G-5): sc-376471 react with Zebrafish samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 13
Date published: 2017-01-13
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Rated 1 out of 5 by from Not specific for FOXM1 - Do not use this antibody! As noticed by other users before, the FOXM1 G-5 antibody detects a protein with the same molecular weight as FOXM1 in Western Blots, but
Date published: 2018-07-18
Rated 4 out of 5 by from Great antibodies I got a free sample from company. I used antibodies in WB in dilution 1:1000. It works good.
Date published: 2018-07-14
Rated 1 out of 5 by from Not sure if observed band is FOXM1 Like the previous comments below, our group has also confirmed that this antibody may not be recognizing FOXM1. We observe a strong clear band at around 125-130 kDa with this antibody in our cell lines. However, upon using both siRNA and shRNA approaches with 3 different species, we could not observe a clear knockdown of FOXM1 with this antibody. In contrast, FOXM1 (A-11) sc-271746 showed knockdown of FOXM1 protein. Additionally, while this antibody can recognize overexpressed FOXM1, the exogenous FOXM1 appears as a second at around 110 kDa. The FOXM1 (A-11) antibody only recognizes a single band with much greater intensity. The FOXM1 (A-11) antibody also gave consistent results with CS #D12D5 and an Abcam antibody.
Date published: 2018-05-11
Rated 3 out of 5 by from Gives good band, but uncertain about specificity The mouse G-5 antibody appears to detect primarily nuclear FOXM1; whereas the SC-500 rabbit antibody detects mainly cytoplasmic (and to some extent nuclear). The expression pattern in western blots, ICC, and IHC have confirmed this (images attached). Possibly due to the G-5 antibody unable to detect FOXM1 in cytoplasm if dimerized? It seems to detect FOXM1 differently in regards to localization; however, the MW appears to be correct. I still haven't quite figured this one out. In any case, I used the antibody at a 1:1000 dilution and secondary at 1:2500. I've noticed most SC antibodies work well at 1:250/500 primary and secondary at 1:2500. It has worked well with overexpression (human FOXM1b isoform) as well as siRNA knockdown in rat cells.
Date published: 2018-03-26
Rated 1 out of 5 by from Not sure the band we detect is FOXM1 We have observed similar results than the one showed by elenaenzo with two different siRNA against FOXM1 in HeLa cells (an antibody against FOXM1 from another firm was used as a positive control on the same lysates).
Date published: 2018-03-16
Rated 2 out of 5 by from no good signal in IF I didn't detect good staining signal in 4%PFA fixed embyronic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 1 out of 5 by from not specific For two times in two different experiments I loaded two different bis-tris gels with the very same extracts from control cells (siCTRL and non-treated cells) and FOXM1 depleted cells with 2 different siRNA and I blotted the membrane with 2 different antibodies, the rabbit anti FOXM1 C-20 and mouse anti-FOXM1 G-5. Unfortunatly, as exemplified in the PDF in attachment, the G-5 antibody seems not specific for endogenous FOXM1 as C-20 antibody.
Date published: 2018-02-16
Rated 5 out of 5 by from wonderful primary antibody 1:200 diluted for western blot of rat protein which extracted from h9c2 cell,the band is stong
Date published: 2018-01-31
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