Flt-4 Antibody (D-6): sc-28297

Flt-4 Antibody (D-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects Flt-4 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Flt-4 (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Flt-4, also known as VEGFR3, is a receptor tyrosine kinase that plays a crucial role in lymphangiogenesis and lymphatic system development. Flt-4 is primarily located on endothelial cell surfaces, where Flt-4 regulates lymphatic vessel formation and maintenance. Proper Flt-4 function is essential for fluid homeostasis and immune response, facilitating immune cell transport and interstitial fluid drainage. Dysregulation of Flt-4 signaling has been implicated in various pathological conditions, including cancer metastasis and lymphedema, making Flt-4 a significant therapeutic target. Seven immunoglobulin-like domains in Flt-4′s structure contribute to high-affinity ligand binding, similar to related receptors Flt-1 and Flk-1, which interact with vascular endothelial growth factors.

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Flt-4 Antibody (D-6) References:

1. Immunolocalisation of the VEGF receptors FLT-1, KDR, and FLT-4 in diabetic retinopathy.  |  Smith, G., et al. 1999. Br J Ophthalmol. 83: 486-94. PMID: 10434875
2. The fms-like tyrosine kinase, a receptor for vascular endothelial growth factor.  |  de Vries, C., et al. 1992. Science. 255: 989-91. PMID: 1312256
3. A receptor tyrosine kinase cDNA isolated from a population of enriched primitive hematopoietic cells and exhibiting close genetic linkage to c-kit.  |  Matthews, W., et al. 1991. Proc Natl Acad Sci U S A. 88: 9026-30. PMID: 1717995
4. Nucleotide sequence and expression of a novel human receptor-type tyrosine kinase gene (flt) closely related to the fms family.  |  Shibuya, M., et al. 1990. Oncogene. 5: 519-24. PMID: 2158038
5. FLT4/VEGFR3 activates AMPK to coordinate glycometabolic reprogramming with autophagy and inflammasome activation for bacterial elimination.  |  Ma, L., et al. 2022. Autophagy. 18: 1385-1400. PMID: 34632918
6. FLT4 as a marker for predicting prognostic risk of refractory acute myeloid leukemia.  |  Lee, JY., et al. 2023. Haematologica. 108: 2933-2945. PMID: 37317880
7. Role and mechanism of FLT4 in high-fat diet-induced obesity in mice.  |  Hao, J., et al. 2023. Biochem Biophys Res Commun. 675: 61-70. PMID: 37451219
8. FLT4 causes developmental disorders of the cardiovascular and lymphovascular systems via pleiotropic molecular mechanisms.  |  Monaghan, RM., et al. 2024. Cardiovasc Res. 120: 1164-1176. PMID: 38713105
9. High affinity VEGF binding and developmental expression suggest Flk-1 as a major regulator of vasculogenesis and angiogenesis.  |  Millauer, B., et al. 1993. Cell. 72: 835-46. PMID: 7681362
10. Vascular endothelial growth factor receptor expression during embryogenesis and tissue repair suggests a role in endothelial differentiation and blood vessel growth.  |  Peters, KG., et al. 1993. Proc Natl Acad Sci U S A. 90: 8915-9. PMID: 7692439
11. The FLT4 gene encodes a transmembrane tyrosine kinase related to the vascular endothelial growth factor receptor.  |  Galland, F., et al. 1993. Oncogene. 8: 1233-40. PMID: 8386825
12. NYK/FLK-1: a putative receptor protein tyrosine kinase isolated from E10 embryonic neuroepithelium is expressed in endothelial cells of the developing embryo.  |  Oelrichs, RB., et al. 1993. Oncogene. 8: 11-8. PMID: 8423988

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Flt-4 Antibody (D-6) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-Flt-4 Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Flt-4 Antibody (D-6) Specifications

Alternate Product Name: VEGFR3 receptor tyrosine kinase

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 8-240

Epitope Target: Flt-4

Epitope Species: human

Product Reactivity/Detection: Flt-4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for Flt-4 Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin