Flightless I Antibody (E-1): sc-55583

Flightless I Antibody (E-1) is a mouse monoclonal IgG2b antibody that detects Flightless I in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Flightless I plays a crucial role in cellular processes, particularly in actin cytoskeleton organization, which is essential for cell migration, division, and shape maintenance. Flightless I contains an Actin-binding domain sharing homology with the Gelsolin family, indicating involvement in actin filament dynamics and rearrangements. Flightless I features an N-terminal leucine-rich repeat domain facilitating protein-protein interactions, further underscoring importance in cellular signaling pathways. Flightless I predominantly localizes in the nucleus, with subsequent translocation to the cytoplasm upon serum stimulation, highlighting dynamic role in cellular responses to external stimuli. In migrating cells, Flightless I co-localizes with structures based on beta-tubulin and actin, emphasizing integral function in coordinating cytoskeletal rearrangements necessary for effective cell movement. The human FLI gene, encoding Flightless I, is located within the Smith-Magenis microdeletion region on chromosome 17 at 17p11.2, and mutations in this gene are associated with Smith-Magenis syndrome, a condition characterized by various developmental and behavioral challenges.

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Flightless I Antibody (E-1) References:

1. Flightless-I (Fli-I) regulates the actin assembly activity of diaphanous-related formins (DRFs) Daam1 and mDia1 in cooperation with active Rho GTPase.  |  Higashi, T., et al. 2010. J Biol Chem. 285: 16231-8. PMID: 20223827
2. Flightless I interacts with NMMIIA to promote cell extension formation, which enables collagen remodeling.  |  Arora, PD., et al. 2015. Mol Biol Cell. 26: 2279-97. PMID: 25877872
3. Flightless I Homolog Represses Prostate Cancer Progression through Targeting Androgen Receptor Signaling.  |  Wang, T., et al. 2016. Clin Cancer Res. 22: 1531-44. PMID: 26527749
4. Flightless-I governs cell fate by recruiting the SUMO isopeptidase SENP3 to distinct HOX genes.  |  Nayak, A., et al. 2017. Epigenetics Chromatin. 10: 15. PMID: 28344658
5. Flightless-I homolog regulates glucocorticoid receptor-mediated transcription via direct interaction of the leucine-rich repeat domain.  |  Jin, HL., et al. 2017. Mol Biol Rep. 44: 243-250. PMID: 28455686
6. Flightless-I Blocks p62-Mediated Recognition of LC3 to Impede Selective Autophagy and Promote Breast Cancer Progression.  |  He, JP., et al. 2018. Cancer Res. 78: 4853-4864. PMID: 29898994
7. Flightless I Alters the Inflammatory Response and Autoantibody Profile in an OVA-Induced Atopic Dermatitis Skin-Like Disease.  |  Kopecki, Z., et al. 2018. Front Immunol. 9: 1833. PMID: 30147695
8. Effect of Flightless I Expression on Epidermal Stem Cell Niche During Wound Repair.  |  Yang, GN., et al. 2020. Adv Wound Care (New Rochelle). 9: 161-173. PMID: 32117580
9. Nucleoredoxin interaction with flightless-I/actin complex is differentially altered in alcoholic liver disease.  |  Alarcón-Sánchez, BR., et al. 2020. Basic Clin Pharmacol Toxicol. 127: 389-404. PMID: 32524749
10. Increased Expression of Flightless I in Cutaneous Squamous Cell Carcinoma Affects Wnt/β-Catenin Signaling Pathway.  |  Yang, GN., et al. 2021. Int J Mol Sci. 22: PMID: 34948000

The preferred Flightless I antibody is Flightless I Antibody (116.40): sc-21716.