Flightless I Antibody (116.40): sc-21716

Flightless I Antibody (116.40) is a mouse monoclonal IgG2a kappa light chain antibody that detects Flightless I protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Flightless I antibody (116.40) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Flightless I protein plays a crucial role in cellular processes, particularly in the organization of the actin cytoskeleton, which is essential for maintaining cell shape, motility, and division. Flightless I contains an Actin-binding domain that shares homology with the gelsolin family, indicating involvement in actin filament dynamics and cellular rearrangements. Flightless I features an N-terminal leucine-rich repeat domain that facilitates protein-protein interactions, further underscoring importance in cellular signaling pathways. Flightless I is predominantly localized in the nucleus but translocates to the cytoplasm upon serum stimulation, where Flightless I co-localizes with beta-tubulin and actin structures in migrating cells. The human FLI gene, which encodes Flightless I protein, is located within the Smith-Magenis microdeletion region on chromosome 17 at 17p11.2, and mutations in this gene are associated with Smith-Magenis syndrome, a condition characterized by various developmental and behavioral challenges.

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Flightless I Antibody (116.40) References:

1. Novel proteins interacting with the leucine-rich repeat domain of human flightless-I identified by the yeast two-hybrid system.  |  Fong, KS. and de Couet, HG. 1999. Genomics. 58: 146-57. PMID: 10366446
2. Fliih, the murine homologue of the Drosophila melanogaster flightless I gene: nucleotide sequence, chromosomal mapping and overlap with Llglh.  |  Campbell, HD., et al. 2000. DNA Seq. 11: 29-40. PMID: 10902907
3. The flightless I protein colocalizes with actin- and microtubule-based structures in motile Swiss 3T3 fibroblasts: evidence for the involvement of PI 3-kinase and Ras-related small GTPases.  |  Davy, DA., et al. 2001. J Cell Sci. 114: 549-62. PMID: 11171324
4. Fliih, a gelsolin-related cytoskeletal regulator essential for early mammalian embryonic development.  |  Campbell, HD., et al. 2002. Mol Cell Biol. 22: 3518-26. PMID: 11971982
5. The flightless I protein and the gelsolin family in nuclear hormone receptor-mediated signalling.  |  Archer, SK., et al. 2004. Biochem Soc Trans. 32: 940-2. PMID: 15506930
6. Flightless I: an actin-remodelling protein and an important negative regulator of wound repair.  |  Kopecki, Z. and Cowin, AJ. 2008. Int J Biochem Cell Biol. 40: 1415-9. PMID: 17526423
7. The Activities of the Gelsolin Homology Domains of Flightless-I in Actin Dynamics.  |  Pintér, R., et al. 2020. Front Mol Biosci. 7: 575077. PMID: 33033719
8. Flightless-I is a potential biomarker for the early detection of alcoholic liver disease.  |  Arellanes-Robledo, J., et al. 2021. Biochem Pharmacol. 183: 114323. PMID: 33166508
9. Multifunctional Roles of the Actin-Binding Protein Flightless I in Inflammation, Cancer and Wound Healing.  |  Strudwick, XL. and Cowin, AJ. 2020. Front Cell Dev Biol. 8: 603508. PMID: 33330501
10. Flightless I Homolog Reverses Enzalutamide Resistance through PD-L1-Mediated Immune Evasion in Prostate Cancer.  |  Ruan, H., et al. 2021. Cancer Immunol Res. 9: 838-852. PMID: 34011528
11. Flightless I is a catabolic factor of chondrocytes that promotes hypertrophy and cartilage degeneration in osteoarthritis.  |  Ebata, T., et al. 2021. iScience. 24: 102643. PMID: 34142066
12. Flightless I Negatively Regulates Macrophage Surface TLR4, Delays Early Inflammation, and Impedes Wound Healing.  |  Mills, SJ., et al. 2022. Cells. 11: PMID: 35883634

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Flightless I Antibody (116.40) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-Flightless I Antibody (116.40) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Flightless I Antibody (116.40) Specifications

Alternate Product Name: Flightless I

Product Clone Name: 116.40

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Flightless I

Epitope Species: human

Product Reactivity/Detection: Flightless I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for Flightless I Antibody (116.40): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin