FKBP12 Antibody (H-5): sc-133067
- FKBP12 Antibody (H-5) is a mouse monoclonal IgG2a κ FKBP12 antibody, cited in 19 publications, provided at 200 µg/ml
- raised against amino acids 1-108 representing full length FKBP12 of human origin
- FKBP12 Antibody (H-5) is recommended for detection of FKBP12 and FKBP12.6 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-FKBP12 Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for FKBP12 Antibody (H-5) for WB applications. These reagents are now offered in bundles with FKBP12 Antibody (H-5) (see ordering information below).
FKBP12 Antibody (H-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects FKBP12 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FKBP12 (H-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FKBP12, also known as 12 KDa FK506-Binding Protein, plays a crucial role in cellular processes by acting as a peptidyl-prolyl isomerase, which facilitates protein folding and regulates function. FKBP12 is primarily located in the cytoplasm, where FKBP12 interacts with various signaling pathways, including those involved in T cell activation and calcium signaling. FKBP12′s cytoplasmic localization enables modulation of key receptors, such as type I TGFβ receptor, thereby influencing cellular responses to growth factors and cytokines. FKBP12 binds immunosuppressive drugs like FK506 and Rapamycin, underscoring significance in pharmacological contexts and potential therapeutic applications.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
FKBP12 Antibody (H-5) References:
- FKBP12, the 12-kDa FK506-binding protein, is a physiologic regulator of the cell cycle. | Aghdasi, B., et al. 2001. Proc Natl Acad Sci U S A. 98: 2425-30. PMID: 11226255
- FKBP12 functions as an adaptor of the Smad7-Smurf1 complex on activin type I receptor. | Yamaguchi, T., et al. 2006. J Mol Endocrinol. 36: 569-79. PMID: 16720724
- FKBP12 binds to acylated H-ras and promotes depalmitoylation. | Ahearn, IM., et al. 2011. Mol Cell. 41: 173-85. PMID: 21255728
- FKBP12.6 activates RyR1: investigating the amino acid residues critical for channel modulation. | Venturi, E., et al. 2014. Biophys J. 106: 824-33. PMID: 24559985
- FKBP12 enhances sensitivity to chemotherapy-induced cancer cell apoptosis by inhibiting MDM2. | Liu, T., et al. 2017. Oncogene. 36: 1678-1686. PMID: 27617579
- FKBP12 is a predictive biomarker for efficacy of anthracycline-based chemotherapy in breast cancer. | Xing, M., et al. 2019. Cancer Chemother Pharmacol. 84: 861-872. PMID: 31428819
- FKBP12 dimerization mutations effect FK506 binding and differentially alter calcineurin inhibition in the human pathogen Aspergillus fumigatus. | Juvvadi, PR., et al. 2020. Biochem Biophys Res Commun. 526: 48-54. PMID: 32192767
- FKBP12 is a major regulator of ALK2 activity in multiple myeloma cells. | Quist-Løkken, I., et al. 2023. Cell Commun Signal. 21: 25. PMID: 36717825
- FKBP12 binds to the cardiac ryanodine receptor with negative cooperativity: implications for heart muscle physiology in health and disease. | Richardson, SJ., et al. 2023. Philos Trans R Soc Lond B Biol Sci. 378: 20220169. PMID: 37122219
- HMGA1 sensitizes esophageal squamous cell carcinoma to mTOR inhibitors through the ETS1-FKBP12 axis. | Guo, JR., et al. 2024. Int J Biol Sci. 20: 2640-2657. PMID: 38725843
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FKBP12 Antibody (H-5) | sc-133067 | 200 µg/ml | $316.00 | |||
FKBP12 Antibody (H-5): m-IgGκ BP-HRP Bundle | sc-521222 | 200 µg Ab, 40 µg BP | $354.00 | |||
FKBP12 Antibody (H-5): m-IgG2a BP-HRP Bundle | sc-546088 | 200 µg Ab; 10 µg BP | $354.00 | |||
FKBP12 Antibody (H-5) AC | sc-133067 AC | 500 µg/ml, 25% agarose | $416.00 | |||
FKBP12 Antibody (H-5) HRP | sc-133067 HRP | 200 µg/ml | $316.00 | |||
FKBP12 Antibody (H-5) FITC | sc-133067 FITC | 200 µg/ml | $330.00 | |||
FKBP12 Antibody (H-5) PE | sc-133067 PE | 200 µg/ml | $343.00 | |||
FKBP12 Antibody (H-5) Alexa Fluor® 488 | sc-133067 AF488 | 200 µg/ml | $357.00 | |||
FKBP12 Antibody (H-5) Alexa Fluor® 546 | sc-133067 AF546 | 200 µg/ml | $357.00 | |||
FKBP12 Antibody (H-5) Alexa Fluor® 594 | sc-133067 AF594 | 200 µg/ml | $357.00 | |||
FKBP12 Antibody (H-5) Alexa Fluor® 647 | sc-133067 AF647 | 200 µg/ml | $357.00 | |||
FKBP12 Antibody (H-5) Alexa Fluor® 680 | sc-133067 AF680 | 200 µg/ml | $357.00 | |||
FKBP12 Antibody (H-5) Alexa Fluor® 790 | sc-133067 AF790 | 200 µg/ml | $357.00 |