FKBP12 Antibody (G-4): sc-136962
- FKBP12 Antibody (G-4) is a mouse monoclonal IgG2b κ, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-108 representing full length FKBP12 of human origin
- Anti-FKBP12 Antibody (G-4) is recommended for detection of FKBP12 and FKBP12.6 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-FKBP12 Antibody (G-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for FKBP12 Antibody (G-4). This work is in progress.
FKBP12 Antibody (G-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects FKBP12 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-FKBP12 antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FKBP12, a member of the immunophilin family, plays a crucial role in cellular processes by acting as a peptidyl-prolyl isomerase, which is essential for protein folding and function. FKBP12 is primarily located in the cytoplasm, where FKBP12 is involved in regulating T cell activation, cell cycle entry, and intracellular calcium release. FKBP12′s cytoplasmic localization enables interaction with various signaling pathways, including constitutive association with the intracellular domain of the type I TGFβ receptor, which is vital for modulating TGFβ signaling independent of receptor activation. This interaction highlights FKBP12′s potential role in maintaining cellular homeostasis and importance in therapeutic contexts, particularly in the modulation of immune responses and cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
FKBP12 Antibody (G-4) References:
- The chemistry of signal transduction. | Clardy, J. 1995. Proc Natl Acad Sci U S A. 92: 56-61. PMID: 7529414
- The complex of FK506-binding protein 12 and FK506 inhibits calcineurin phosphatase activity and IgE activation-induced cytokine transcripts, but not exocytosis, in mouse mast cells. | Fruman, DA., et al. 1995. J Immunol. 154: 1846-51. PMID: 7530743
- Control of p70 s6 kinase by kinase activity of FRAP in vivo. | Brown, EJ., et al. 1995. Nature. 377: 441-6. PMID: 7566123
- Molecular mechanisms of immunosuppression by cyclosporine, FK506, and rapamycin. | Cardenas, ME., et al. 1995. Curr Opin Nephrol Hypertens. 4: 472-7. PMID: 8591053
- Structure of the FKBP12-rapamycin complex interacting with the binding domain of human FRAP. | Choi, J., et al. 1996. Science. 273: 239-42. PMID: 8662507
- Characterization of the interaction of FKBP12 with the transforming growth factor-beta type I receptor in vivo. | Okadome, T., et al. 1996. J Biol Chem. 271: 21687-90. PMID: 8702959
- The immunophilin FKBP12 functions as a common inhibitor of the TGF beta family type I receptors. | Wang, T., et al. 1996. Cell. 86: 435-44. PMID: 8756725
- Cellular functions of immunophilins. | Marks, AR. 1996. Physiol Rev. 76: 631-49. PMID: 8757784
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
FKBP12 Antibody (G-4) | sc-136962 | 200 µg/ml | $316.00 | |||
FKBP12 Antibody (G-4) AC | sc-136962 AC | 500 µg/ml, 25% agarose | $416.00 | |||
FKBP12 Antibody (G-4) HRP | sc-136962 HRP | 200 µg/ml | $316.00 | |||
FKBP12 Antibody (G-4) FITC | sc-136962 FITC | 200 µg/ml | $330.00 | |||
FKBP12 Antibody (G-4) PE | sc-136962 PE | 200 µg/ml | $343.00 | |||
FKBP12 Antibody (G-4) Alexa Fluor® 488 | sc-136962 AF488 | 200 µg/ml | $357.00 | |||
FKBP12 Antibody (G-4) Alexa Fluor® 546 | sc-136962 AF546 | 200 µg/ml | $357.00 | |||
FKBP12 Antibody (G-4) Alexa Fluor® 594 | sc-136962 AF594 | 200 µg/ml | $357.00 | |||
FKBP12 Antibody (G-4) Alexa Fluor® 647 | sc-136962 AF647 | 200 µg/ml | $357.00 | |||
FKBP12 Antibody (G-4) Alexa Fluor® 680 | sc-136962 AF680 | 200 µg/ml | $357.00 | |||
FKBP12 Antibody (G-4) Alexa Fluor® 790 | sc-136962 AF790 | 200 µg/ml | $357.00 |