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Filamin 3 Antibody (D-10) - Western Blotting - Image 319560
Filamin 3 Antibody (D-10): sc-271561. Western blot analysis of Filamin 3 expression in K-562 (A) and U-87 MG (B) whole cell lysates.
Filamin 3 Antibody (D-10): sc-271561. Western blot analysis of Filamin 3 expression in K-562 (A) and U-87 MG (B) whole cell lysates.

Filamin 3 Antibody (D-10): sc-271561

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Datasheets
  • Filamin 3 Antibody (D-10) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 386-450 mapping within an internal region of Filamin 3 of human origin
  • recommended for detection of Filamin 3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Filamin 3 Antibody (D-10) for WB and IHC(P) applications. These reagents are now offered in bundles with Filamin 3 Antibody (D-10) (see ordering information below).
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Additional Filamin Antibodies including Filamin 1

Filamin 3 Antibody (D-10) is a mouse monoclonal IgG1 antibody that detects Filamin 3 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Filamin 3, also known as Filamin B or β-Filamin, maintains structural integrity of the cytoskeleton by forming flexible cross-links between actin filaments, essential for cellular shape and mechanical stability. Filamin 3 predominantly resides in the cytoplasm and plays key roles during endochondral ossification, vertebral segmentation, and joint formation. Filamin 3 facilitates neuroblast migration into the cortical plate from the ventricular zone, a critical process for brain development. Mutations in FLNB, the gene encoding Filamin 3, have been linked to several skeletal disorders, including autosomal dominant Larsen syndrome and spondylocarpotarsal syndrome, as well as periventricular heterotopia, highlighting Filamin 3′s importance in both skeletal and neural development. Anti-Filamin 3 antibody (D-10) provides researchers with a reliable tool for studying these pathways and disorders, enabling deeper understanding of Filamin 3′s multifaceted roles in cellular architecture and function.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Filamin 3 Antibody (D-10) References:

  1. Filamin B represses chondrocyte hypertrophy in a Runx2/Smad3-dependent manner.  |  Zheng, L., et al. 2007. J Cell Biol. 178: 121-8. PMID: 17606870
  2. Filamin B mediates ICAM-1-driven leukocyte transendothelial migration.  |  Kanters, E., et al. 2008. J Biol Chem. 283: 31830-9. PMID: 18809679
  3. ISG15 modification of filamin B negatively regulates the type I interferon-induced JNK signalling pathway.  |  Jeon, YJ., et al. 2009. EMBO Rep. 10: 374-80. PMID: 19270716
  4. The filamin-B-refilin axis - spatiotemporal regulators of the actin-cytoskeleton in development and disease.  |  Baudier, J., et al. 2018. J Cell Sci. 131: PMID: 29654161
  5. Organ-wide profiling in mouse reveals high editing levels of Filamin B mRNA in the musculoskeletal system.  |  Czermak, P., et al. 2018. RNA Biol. 15: 877-885. PMID: 30064337
  6. Decreased Filamin b expression regulates trophoblastic cells invasion through ERK/MMP-9 pathway in pre-eclampsia.  |  Wei, J., et al. 2019. Ginekol Pol. 90: 39-45. PMID: 30756369
  7. Intervertebral disc degeneration is rescued by TGFβ/BMP signaling modulation in an ex vivo filamin B mouse model.  |  Zieba, J., et al. 2022. Bone Res. 10: 37. PMID: 35474298
  8. Cell-Dependent Pathogenic Roles of Filamin B in Different Skeletal Malformations.  |  Wu, H., et al. 2022. Oxid Med Cell Longev. 2022: 8956636. PMID: 35832491
  9. Actin-binding protein filamin B regulates the cell-surface retention of endothelial sphingosine 1-phosphate receptor 1.  |  Zhao, X., et al. 2023. J Biol Chem. 299: 104851. PMID: 37220855
  10. Filamin B restricts vaccinia virus spread and is targeted by vaccinia virus protein C4.  |  Georgana, I., et al. 2024. J Virol. 98: e0148523. PMID: 38412044

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Filamin 3 Antibody (D-10)

sc-271561
200 µg/ml
$322.00

Filamin 3 Antibody (D-10): m-IgG Fc BP-HRP Bundle

sc-537662
200 µg Ab; 10 µg BP
$361.00

Filamin 3 Antibody (D-10): m-IgGκ BP-HRP Bundle

sc-534997
200 µg Ab; 40 µg BP
$361.00

Filamin 3 Antibody (D-10): m-IgG1 BP-HRP Bundle

sc-545291
200 µg Ab; 20 µg BP
$361.00

Can Filamin 3 (D-10): sc-271561 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, Filamin 3 (D-10): sc-271561 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic stainingSuperb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human colon tissue. -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by from Positive results in western blot analysisPositive results in western blot analysis of Filamin 3 expression in K-562 and U-87 MG whole cell lysates. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by from Robust band in western blot analysis usingRobust band in western blot analysis using Jurkat whole cell lysate. -SCBT QC
Date published: 2015-02-23
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Filamin 3 Antibody (D-10) is rated 5.0 out of 5 by 3.
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