Fibulin-3 Antibody (mab3-5): sc-33722

Fibulin-3 Antibody (mab3-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects Fibulin-3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Fibulin-3, also known as EFEMP1, is a secreted protein that plays a crucial role in the structural integrity of the extracellular matrix, particularly in the formation of elastic fibers. Proper Fibulin-3 function is essential for maintaining tissue elasticity and resilience, which is vital for organs that undergo significant mechanical stress, such as the lungs and skin. Mutations in the gene encoding Fibulin-3 are linked to Doyne honeycomb retinal dystrophy, a genetic condition characterized by the accumulation of yellow-white deposits beneath the retinal pigment epithelium, leading to vision impairment. Anti-Fibulin-3 antibody (mab3-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Fibulin-3 Antibody (mab3-5) References:

1. A single EFEMP1 mutation associated with both Malattia Leventinese and Doyne honeycomb retinal dystrophy.  |  Stone, EM., et al. 1999. Nat Genet. 22: 199-202. PMID: 10369267
2. Sequence, recombinant expression and tissue localization of two novel extracellular matrix proteins, fibulin-3 and fibulin-4.  |  Giltay, R., et al. 1999. Matrix Biol. 18: 469-80. PMID: 10601734
3. Dominant radial drusen and Arg345Trp EFEMP1 mutation.  |  Matsumoto, M. and Traboulsi, EI. 2001. Am J Ophthalmol. 131: 810-2. PMID: 11384588
4. Aberrant accumulation of EFEMP1 underlies drusen formation in Malattia Leventinese and age-related macular degeneration.  |  Marmorstein, LY., et al. 2002. Proc Natl Acad Sci U S A. 99: 13067-72. PMID: 12242346
5. Tissue inhibitor of metalloproteinases-3 (TIMP-3) is a binding partner of epithelial growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1). Implications for macular degenerations.  |  Klenotic, PA., et al. 2004. J Biol Chem. 279: 30469-73. PMID: 15123717
6. Analysis of the EFEMP1 gene in individuals and families with early onset drusen.  |  Narendran, N., et al. 2005. Eye (Lond). 19: 11-5. PMID: 15218514
7. Association of EFEMP1 with malattia leventinese and age-related macular degeneration: a mini-review.  |  Marmorstein, L. 2004. Ophthalmic Genet. 25: 219-26. PMID: 15512998
8. Mapping wild-type and R345W fibulin-3 intracellular interactomes.  |  Hulleman, JD., et al. 2016. Exp Eye Res. 153: 165-169. PMID: 27777122
9. Fibulin-3 as biomarker of malignant mesothelioma.  |  Ledda, C., et al. 2019. Biomark Med. 13: 875-886. PMID: 31237454
10. The Pathophysiological Significance of Fibulin-3.  |  Livingstone, I., et al. 2020. Biomolecules. 10: PMID: 32911658
11. Fibulin-3 is necessary to prevent cardiac rupture following myocardial infarction.  |  Murtha, LA., et al. 2023. Sci Rep. 13: 14995. PMID: 37696945
12. Structure and chromosomal assignment of the human S1-5 gene (FBNL) that is highly homologous to fibrillin.  |  Ikegawa, S., et al. 1996. Genomics. 35: 590-2. PMID: 8812496

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Fibulin-3 Antibody (mab3-5) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-Fibulin-3 Antibody (mab3-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Fibulin-3 Antibody (mab3-5) Specifications

Alternate Product Name: Fibulin-3

Product Clone Name: mab3-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 107-493

Epitope Target: Fibulin-3

Epitope Species: human

Product Reactivity/Detection: precursor and mature Fibulin-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for Fibulin-3 Antibody (mab3-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Fibulin-3 antibody is Fibulin-3 Antibody (C-3): sc-365224.