Fibrillarin Antibody (G-8): sc-374022

Fibrillarin Antibody (G-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Fibrillarin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Fibrillarin (G-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Fibrillarin is a crucial nonhistone protein predominantly localized in the fibrillar region of the nucleolus, specifically within the dense fibrillar and fibrillar center regions, where Fibrillarin plays a vital role in processing ribosomal RNA (rRNA) transcripts. This localization underscores Fibrillarin′s involvement in ribosome biogenesis, a fundamental process for cellular function and growth. Fibrillarin expression occurs in various cell types, including HeLa cells, 3T3 cells, and human peripheral blood lymphocytes, and during cell division, Fibrillarin is found at chromosomal nucleolar regions in metaphase and anaphase, serving as an early marker for nucleolus formation during telophase. Fibrillarin′s structure features an RNA-binding domain and an RNP consensus sequence, which facilitates interaction with U3 small nucleolar RNA, further emphasizing Fibrillarin′s essential role in rRNA processing and ribosome assembly.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Fibrillarin Antibody (G-8) References:

1. Fibrillarin is essential for early development and required for accumulation of an intron-encoded small nucleolar RNA in the mouse.  |  Newton, K., et al. 2003. Mol Cell Biol. 23: 8519-27. PMID: 14612397
2. Fibrillarin, a nucleolar protein, is required for normal nuclear morphology and cellular growth in HeLa cells.  |  Amin, MA., et al. 2007. Biochem Biophys Res Commun. 360: 320-6. PMID: 17603021
3. Evolutionary conservation of the human nucleolar protein fibrillarin and its functional expression in yeast.  |  Jansen, RP., et al. 1991. J Cell Biol. 113: 715-29. PMID: 2026646
4. Fibrillarin: a new protein of the nucleolus identified by autoimmune sera.  |  Ochs, RL., et al. 1985. Biol Cell. 54: 123-33. PMID: 2933102
5. SIRT7-Dependent Deacetylation of Fibrillarin Controls Histone H2A Methylation and rRNA Synthesis during the Cell Cycle.  |  Iyer-Bierhoff, A., et al. 2018. Cell Rep. 25: 2946-2954.e5. PMID: 30540930
6. Discovery of Cofactor Competitive Inhibitors against the Human Methyltransferase Fibrillarin.  |  Shi, Y., et al. 2021. Pharmaceuticals (Basel). 15: PMID: 35056083
7. RNA 2'-O-Methyltransferase Fibrillarin Facilitates Virus Entry Into Macrophages Through Inhibiting Type I Interferon Response.  |  Li, P., et al. 2022. Front Immunol. 13: 793582. PMID: 35464456
8. Low level of Fibrillarin, a ribosome biogenesis factor, is a new independent marker of poor outcome in breast cancer.  |  Nguyen Van Long, F., et al. 2022. BMC Cancer. 22: 526. PMID: 35545761
9. A Viral Suppressor of RNA Silencing May Be Targeting a Plant Defence Pathway Involving Fibrillarin.  |  Pérez-Cañamás, M., et al. 2022. Plants (Basel). 11: PMID: 35893605
10. Fibrillarin, a conserved pre-ribosomal RNA processing protein of Giardia.  |  Narcisi, EM., et al. 1998. J Eukaryot Microbiol. 45: 105-11. PMID: 9495039

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Fibrillarin Antibody (G-8) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-Fibrillarin Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Fibrillarin Antibody (G-8) Specifications

Alternate Product Name: Fibrillarin

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 99-127

Epitope Target: Fibrillarin

Epitope Species: human

Product Reactivity/Detection: Fibrillarin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for Fibrillarin Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Fibrillarin antibody is Fibrillarin Antibody (B-1): sc-166001.