fetuin-A Antibody (H-4): sc-133146

fetuin-A Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects human fetuin-A by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-fetuin-A antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Fetuin-A, also known as alpha-2-HS-glycoprotein or alpha-2-Z-globulin, is a secreted plasma protein primarily produced in the liver, monocytes/macrophages, and bone tissue. Fetuin-A binds and transports calcium and barium ions in the bloodstream, playing a significant part in maintaining serum calcium homeostasis and mineralization. Additionally, fetuin-A acts as an acute phase anti-inflammatory mediator, regulating innate immune response following tissue injury. During inflammatory responses, circulating fetuin-A levels decrease significantly as fetuin-A associates with macrophage membranes, where fetuin-A enhances the uptake of cationic small molecules into activated macrophages, facilitating mechanisms that deactivate these immune cells. A biologically active form of fetuin-A is generated from a precursor protein that undergoes cleavage at the amino terminus, resulting in two chains linked by a single disulfide bond, underscoring the importance of structural integrity for functional roles in health and disease.

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fetuin-A Antibody (H-4) References:

1. Clinical implications of fetuin-A.  |  Jirak, P., et al. 2019. Adv Clin Chem. 89: 79-130. PMID: 30797472
2. Molecular and pathobiological involvement of fetuin-A in the pathogenesis of NAFLD.  |  Sardana, O., et al. 2021. Inflammopharmacology. 29: 1061-1074. PMID: 34185201
3. Fetuin-A Deficiency but Not Pentraxin 3, FGF-21, or Irisin, Predisposes to More Serious COVID-19 Course.  |  Kukla, M., et al. 2021. Biomolecules. 11: PMID: 34680053
4. Human alpha 2-HS-glycoprotein: the A and B chains with a connecting sequence are encoded by a single mRNA transcript.  |  Lee, CC., et al. 1987. Proc Natl Acad Sci U S A. 84: 4403-7. PMID: 3474608
5. Biomarkers in Cardiovascular Disease: The Role of Fetuin-A.  |  Pagan, LU., et al. 2022. Arq Bras Cardiol. 118: 22-23. PMID: 35195204
6. Fetuin-A alleviates neuroinflammation against traumatic brain injury-induced microglial necroptosis by regulating Nrf-2/HO-1 pathway.  |  Zhao, P., et al. 2022. J Neuroinflammation. 19: 269. PMID: 36333786
7. The serum protein alpha2-HS glycoprotein/fetuin inhibits apatite formation in vitro and in mineralizing calvaria cells. A possible role in mineralization and calcium homeostasis.  |  Schinke, T., et al. 1996. J Biol Chem. 271: 20789-96. PMID: 8702833
8. Serum concentration of human alpha 2 HS glycoprotein during the inflammatory process: evidence that alpha 2 HS glycoprotein is a negative acute-phase reactant.  |  Lebreton, JP., et al. 1979. J Clin Invest. 64: 1118-29. PMID: 90057
9. Structure of the gene encoding human alpha 2-HS glycoprotein (AHSG).  |  Osawa, M., et al. 1997. Gene. 196: 121-5. PMID: 9322749
10. Modification of macrophage response to lipopolysaccharide by fetuin.  |  Dziegielewska, KM., et al. 1998. Immunol Lett. 60: 31-5. PMID: 9541460
11. Structural and functional analysis of the 5'-transcription control region for the human alpha2-HS glycoprotein gene.  |  Banine, F., et al. 1998. Biochim Biophys Acta. 1398: 1-8. PMID: 9602019
12. Fetuin (alpha2-HS-glycoprotein) opsonizes cationic macrophagedeactivating molecules.  |  Wang, H., et al. 1998. Proc Natl Acad Sci U S A. 95: 14429-34. PMID: 9826717

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-fetuin-A Antibody (H-4) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-fetuin-A Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

fetuin-A Antibody (H-4) Specifications

Alternate Product Name: AHSG

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 68-367

Epitope Target: fetuin-A

Epitope Species: human

Product Reactivity/Detection: fetuin-A

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for fetuin-A Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin