Fc ε RIγ Antibody (A-5): sc-374335

Fc ε RIγ Antibody (A-5) is a mouse monoclonal IgG2a antibody that detects Fc ε RIγ in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Fc ε RIγ antibody (A-5) specifically targets the gamma chain of the high-affinity IgE receptor, which plays a crucial role in mediating allergic responses. Fc ε RIγ (A-5) antibody recognizes a protein primarily located on the surface of mast cells and basophils, where Fc ε RIγ is essential for the initiation of allergic reactions. Expression of Fc ε RIγ on these immune cells allows for the binding of allergen-specific IgE, leading to cell activation and the subsequent release of inflammatory mediators such as histamines and cytokines. Localization of Fc ε RIγ enables rapid responses to allergens, contributing to the body′s defense mechanisms. The gamma chain serves as a common subunit among various Fc receptors, highlighting Fc ε RIγ importance in immune signaling pathways. Cytoplasmic domains of the beta and gamma subunits contain immunoreceptor tyrosine activation motifs (ITAMs), which are critical for signal transduction, as phosphorylation of specific tyrosine residues within these motifs is necessary for effective activation of mast cells and other hematopoietic cells. Fc ε RIγ functions as a key player in allergic reactions and serves as a significant target for therapeutic interventions aimed at modulating immune responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Fc ε RIγ Antibody (A-5) References:

1. FcRgamma-chain-dependent alphaIIbeta3 elicited outside-in signaling.  |  Liu, J., et al. 2007. J Thromb Haemost. 5: 426-8. PMID: 17105578
2. Critical role of the Fc receptor gamma-chain on APCs in the development of allergen-induced airway hyperresponsiveness and inflammation.  |  Kitamura, K., et al. 2007. J Immunol. 178: 480-8. PMID: 17182587
3. The expanding roles of ITAM adapters FcRgamma and DAP12 in myeloid cells.  |  Hamerman, JA., et al. 2009. Immunol Rev. 232: 42-58. PMID: 19909355
4. FcRγ promotes T cell apoptosis in Fas-deficient mice.  |  Juvet, SC., et al. 2013. J Autoimmun. 42: 80-93. PMID: 23313147
5. Type II phosphatidylinositol 4-kinases interact with FcεRIγ subunit in RBL-2H3 cells.  |  Bojjireddy, N., et al. 2014. Mol Cell Biochem. 390: 197-203. PMID: 24481753
6. Regulation of FcRγ function by site-specific serine phosphorylation.  |  Shah, S., et al. 2017. J Leukoc Biol. 101: 421-428. PMID: 27630214
7. FcRγ deficiency improves survival in experimental sepsis by down-regulating TLR4 signaling pathway.  |  Wei, ZM., et al. 2019. Immunol Res. 67: 77-83. PMID: 30552619
8. Diminished cell proliferation promotes natural killer cell adaptive-like phenotype by limiting FcεRIγ expression.  |  Shemesh, A., et al. 2022. J Exp Med. 219: PMID: 36066491
9. The kinetics of signaling through the common FcRγ chain determine cytokine profiles in dendritic cells.  |  Watanabe, M., et al. 2023. Sci Signal. 16: eabn9909. PMID: 36881655
10. Purification and characterisation of the platelet-activating GPVI/FcRγ complex in SMALPs.  |  Wang, X., et al. 2024. Arch Biochem Biophys. 754: 109944. PMID: 38395124

The preferred Fc ε RIγ antibody is Fc ε RIγ Antibody (E-12): sc-390222.