Fc ε RIβ Antibody (H-5): sc-398863
- Fc ε RIβ Antibody (H-5) is a mouse monoclonal IgG2b κ, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 111-235 mapping at the C-terminus of Fc ε RIβ of mouse origin
- Anti-Fc epsilon RI beta Antibody (H-5) is recommended for detection of Fc ε RIβ of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Fc epsilon RI beta Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Fc ε RIβ Antibody (H-5) for WB applications. These reagents are now offered in bundles with Fc ε RIβ Antibody (H-5) (see ordering information below).
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Fc ε RIβ Antibody (H-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects Fc ε RIβ of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Fc ε RIβ (H-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IgE Fc Receptor I plays a crucial role in immune response by binding with high affinity to immunoglobulin epsilon chains′ Fc region, which is essential for initiating allergic reactions. Upon allergen binding to receptor-bound IgE, cellular activation occurs, leading to histamine release responsible for allergy symptoms. Additionally, IgE Fc Receptor I induces lymphokine secretion vital for hypersensitivity response. Structurally, IgE Fc Receptor I forms a tetramer composed of a heavily glycosylated alpha chain, a beta chain, and two disulfide-linked gamma chains. Beta chain features four transmembrane regions with long cytoplasmic domains involved in intracellular signaling. Notably, beta and gamma subunits′ cytoplasmic domains contain a conserved immunoreceptor tyrosine activation motif (ITAM), where specific tyrosine residue phosphorylation is critical for signal transduction in mast cells and other hematopoietic cell types. A variant at Glu-237 of beta subunit has been associated with increased atopic dermatitis and asthma risk, highlighting Fc ε RIβ′s importance in allergic diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Fc ε RIβ Antibody (H-5) References:
- A second amplifier function for the allergy-associated Fc(epsilon)RI-beta subunit. | Donnadieu, E., et al. 2000. Immunity. 12: 515-23. PMID: 10843384
- Fc epsilon R1-mediated tyrosine phosphorylation of multiple proteins, including phospholipase C gamma 1 and the receptor beta gamma 2 complex, in RBL-2H3 rat basophilic leukemia cells. | Li, W., et al. 1992. Mol Cell Biol. 12: 3176-82. PMID: 1535686
- IgE signaling suppresses FcepsilonRIbeta expression. | Brenzovich, J., et al. 2009. J Leukoc Biol. 86: 1351-8. PMID: 19741159
- Fine-Tuning of Mast Cell Activation by FcεRIβ Chain. | Ra, C., et al. 2012. Front Immunol. 3: 112. PMID: 22623922
- Association between FcεRIβ and IFN-γ Polymorphisms and Asthma in Asian Population: a Meta-Analysis. | Yao, YS., et al. 2015. Iran J Allergy Asthma Immunol. 14: 1-11. PMID: 25530133
- Analysis of the conformation and thermal stability of the high-affinity IgE Fc receptor β chain polymorphic proteins. | Terada, T., et al. 2016. Biosci Biotechnol Biochem. 80: 1356-61. PMID: 26940508
- Exon Skipping of FcεRIβ for Allergic Diseases. | Arthur, GK. and Cruse, G. 2018. Methods Mol Biol. 1828: 503-518. PMID: 30171564
- Regulation of Trafficking and Signaling of the High Affinity IgE Receptor by FcεRIβ and the Potential Impact of FcεRIβ Splicing in Allergic Inflammation. | Arthur, GK. and Cruse, G. 2022. Int J Mol Sci. 23: PMID: 35054974
- Effect of saiboku-to (TJ-96) on bronchial asthma. Induction of glucocorticoid receptor, beta-adrenaline receptor, IgE-Fc epsilon receptor expression and its effect on experimental immediate and late asthmatic reaction. | Nakajima, S., et al. 1993. Ann N Y Acad Sci. 685: 549-60. PMID: 8395780
- The Fc(epsilon)RIbeta subunit functions as an amplifier of Fc(epsilon)RIgamma-mediated cell activation signals. | Lin, S., et al. 1996. Cell. 85: 985-95. PMID: 8674126
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Fc ε RIβ Antibody (H-5) | sc-398863 | 200 µg/ml | $322.00 | |||
Fc ε RIβ Antibody (H-5): m-IgG Fc BP-HRP Bundle | sc-530946 | 200 µg Ab; 10 µg BP | $361.00 | |||
Fc ε RIβ Antibody (H-5): m-IgGκ BP-HRP Bundle | sc-524511 | 200 µg Ab, 40 µg BP | $361.00 | |||
Fc ε RIβ Antibody (H-5) AC | sc-398863 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Fc ε RIβ Antibody (H-5) HRP | sc-398863 HRP | 200 µg/ml | $322.00 | |||
Fc ε RIβ Antibody (H-5) FITC | sc-398863 FITC | 200 µg/ml | $336.00 | |||
Fc ε RIβ Antibody (H-5) PE | sc-398863 PE | 200 µg/ml | $349.00 | |||
Fc ε RIβ Antibody (H-5) Alexa Fluor® 488 | sc-398863 AF488 | 200 µg/ml | $364.00 | |||
Fc ε RIβ Antibody (H-5) Alexa Fluor® 546 | sc-398863 AF546 | 200 µg/ml | $364.00 | |||
Fc ε RIβ Antibody (H-5) Alexa Fluor® 594 | sc-398863 AF594 | 200 µg/ml | $364.00 | |||
Fc ε RIβ Antibody (H-5) Alexa Fluor® 647 | sc-398863 AF647 | 200 µg/ml | $364.00 | |||
Fc ε RIβ Antibody (H-5) Alexa Fluor® 680 | sc-398863 AF680 | 200 µg/ml | $364.00 | |||
Fc ε RIβ Antibody (H-5) Alexa Fluor® 790 | sc-398863 AF790 | 200 µg/ml | $364.00 |