Fc ε RIβ Antibody (F-1): sc-393789

Fc ε RIβ Antibody (F-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects Fc ε RIβ of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Fc ε RIβ Antibody (F-1) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IgE Fc Receptor I plays a crucial role in immune response by binding with high affinity to immunoglobulin epsilon chains′ Fc region, which initiates allergic reactions. Upon allergen binding to receptor-bound IgE, cellular activation triggers release of mediators like histamines responsible for allergy symptoms. This receptor induces lymphokine secretion vital for hypersensitivity response. Structurally, Fc ε RIβ forms a tetramer with a heavily glycosylated alpha chain, beta chain, and two disulfide-linked gamma chains. Beta chain contains four transmembrane regions and long cytoplasmic domains involved in intracellular signaling pathways. Cytoplasmic domains of beta and gamma subunits contain a conserved immunoreceptor tyrosine activation motif (ITAM), where specific tyrosine residue phosphorylation drives signal transduction in mast cells and other hematopoietic cells. A variant at Glu-237 of beta subunit has been linked to increased atopic dermatitis and asthma risk, highlighting Fc ε RIβ significance in allergic diseases.

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Fc ε RIβ Antibody (F-1) References:

1. Identification of a new multigene four-transmembrane family (MS4A) related to CD20, HTm4 and beta subunit of the high-affinity IgE receptor.  |  Ishibashi, K., et al. 2001. Gene. 264: 87-93. PMID: 11245982
2. Determination of the sequence coding for the beta subunit of the human high-affinity IgE receptor.  |  Maekawa, K., et al. 1992. FEBS Lett. 302: 161-5. PMID: 1386024
3. Mast cells, Fc epsilon RI, and IL-13 are required for development of airway hyperresponsiveness after aerosolized allergen exposure in the absence of adjuvant.  |  Taube, C., et al. 2004. J Immunol. 172: 6398-406. PMID: 15128831
4. The beta subunit of the Fc epsilon RI is associated with the Fc gamma RIII on mast cells.  |  Kurosaki, T., et al. 1992. J Exp Med. 175: 447-51. PMID: 1531062
5. Basophil CD63 expression assay on highly sensitized atopic donor leucocytes-a useful method in chronic autoimmune urticaria.  |  Gyimesi, E., et al. 2004. Br J Dermatol. 151: 388-96. PMID: 15327546
6. The gene and cDNA for the human high affinity immunoglobulin E receptor beta chain and expression of the complete human receptor.  |  Küster, H., et al. 1992. J Biol Chem. 267: 12782-7. PMID: 1535625
7. The human genes for the alpha and gamma subunits of the mast cell receptor for immunoglobulin E are located on human chromosome band 1q23.  |  Le Coniat, M., et al. 1990. Immunogenetics. 32: 183-6. PMID: 2146219
8. Human and rat mast cell high-affinity immunoglobulin E receptors: characterization of putative alpha-chain gene products.  |  Shimizu, A., et al. 1988. Proc Natl Acad Sci U S A. 85: 1907-11. PMID: 2964640
9. [Foreign body as cause of a large urethral calculus and diverticulum formation].  |  Hackel, W. 1968. Z Urol Nephrol. 61: 827-9. PMID: 4978125
10. Temporal activation of nontransmembrane protein-tyrosine kinases following mast cell Fc epsilon RI engagement.  |  Penhallow, RC., et al. 1995. J Biol Chem. 270: 23362-5. PMID: 7559493
11. Localisation of atopy and beta subunit of high-affinity IgE receptor (Fc epsilon RI) on chromosome 11q.  |  Sandford, AJ., et al. 1993. Lancet. 341: 332-4. PMID: 8094113
12. Isolation and characterization of the rat beta-subunit gene of the high affinity receptor for immunoglobulin E.  |  Witthuhn, BA. and Faust, C. 1996. Mol Immunol. 33: 881-90. PMID: 8845020

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Fc ε RIβ Antibody (F-1) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Fc ε RIβ Antibody (F-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Fc ε RIβ Antibody (F-1) Specifications

Alternate Product Name: Fc ε RIβ

Product Clone Name: F-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-21

Epitope Target: Fc ε RIβ

Epitope Species: mouse

Product Reactivity/Detection: Fc ε RIβ

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Fc ε RIβ Antibody (F-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin