FBLIM1 Antibody (G-7): sc-271417

FBLIM1 Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects FBLIM1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FBLIM1 (G-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FBLIM1, also known as filamin binding LIM protein 1, CAL (CSX-associated LIM), Mig-2-interacting protein, or Migfilin, is a cytoplasmic protein that plays a crucial role in cellular architecture and signaling. FBLIM1 is primarily located at cell-matrix adhesion sites, where FBLIM1 serves as a scaffold protein, linking the actin cytoskeleton to cell adhesion structures. FBLIM1 contains a proline-rich domain and two to three C-terminal LIM zinc-binding domains, which facilitate interactions with other proteins, including Filamin 3 and Mig-2. These interactions are vital for maintaining the integrity of the cytoskeleton and regulating cellular responses to mechanical stress. Additionally, FBLIM1 can translocate to the nucleus, where FBLIM1 influences gene expression, further underscoring its importance in cellular function and signaling pathways.

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FBLIM1 Antibody (G-7) References:

1. A new member of the LIM protein family binds to filamin B and localizes at stress fibers.  |  Takafuta, T., et al. 2003. J Biol Chem. 278: 12175-81. PMID: 12496242
2. Migfilin and Mig-2 link focal adhesions to filamin and the actin cytoskeleton and function in cell shape modulation.  |  Tu, Y., et al. 2003. Cell. 113: 37-47. PMID: 12679033
3. Physical and functional association of migfilin with cell-cell adhesions.  |  Gkretsi, V., et al. 2005. J Cell Sci. 118: 697-710. PMID: 15671069
4. Migfilin and its binding partners: from cell biology to human diseases.  |  Wu, C. 2005. J Cell Sci. 118: 659-64. PMID: 15701922
5. Migfilin interacts with vasodilator-stimulated phosphoprotein (VASP) and regulates VASP localization to cell-matrix adhesions and migration.  |  Zhang, Y., et al. 2006. J Biol Chem. 281: 12397-407. PMID: 16531412
6. Use of an aggressive MCF-7 cell line variant, TMX2-28, to study cell invasion in breast cancer.  |  Gozgit, JM., et al. 2006. Mol Cancer Res. 4: 905-13. PMID: 17189381
7. Increased cytoplasmic level of migfilin is associated with higher grades of human leiomyosarcoma.  |  Papachristou, DJ., et al. 2007. Histopathology. 51: 499-508. PMID: 17711449
8. FBLIM1 enhances oral cancer malignancy via modulation of the epidermal growth factor receptor pathway.  |  Toeda, Y., et al. 2018. Mol Carcinog. 57: 1690-1697. PMID: 30129678
9. Genetic variants in FBLIM1 gene do not contribute to SAPHO syndrome and chronic recurrent multifocal osteomyelitis in typical patient groups.  |  Assmann, G., et al. 2020. BMC Med Genet. 21: 102. PMID: 32397996
10. Genetics of chronic nonbacterial osteomyelitis in the irish population: no significant association with rare FBLIM1 variants.  |  O'Leary, D., et al. 2021. Pediatr Rheumatol Online J. 19: 32. PMID: 33731121
11. Integrative analyses of bulk and single-cell RNA-seq identified cancer-associated fibroblasts-related signature as a prognostic factor for immunotherapy in NSCLC.  |  Wang, S., et al. 2023. Cancer Immunol Immunother. 72: 2423-2442. PMID: 37010552
12. FBLIM1 mRNA is a novel prognostic biomarker and is associated with immune infiltrates in glioma.  |  Deng, Y., et al. 2023. Open Med (Wars). 18: 20230863. PMID: 38152333

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FBLIM1 Antibody (G-7) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-FBLIM1 Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FBLIM1 Antibody (G-7) Specifications

Alternate Product Name: FBLIM1

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 301-373

Epitope Target: FBLIM1

Epitope Species: human

Product Reactivity/Detection: FBLIM1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for FBLIM1 Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin