Fatso Antibody (C-3): sc-271713

Fatso Antibody (C-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Fatso protein in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Fatso (C-3) antibody comes in non-conjugated form and various conjugates including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® options. Fatso protein, also known as FTO or KIAA1752, is a 505 amino acid protein with an N-terminal nuclear localization signal that guides Fatso to the nucleus for regulating energy homeostasis and fat metabolism. Fatso (C-3) monoclonal antibody recognizes a protein associated with obesity and metabolic disorders, as Fatso gene variants link to increased body mass index and type 2 diabetes risk. Fatso protein shows highest expression in brain and pancreatic tissue, existing in four alternatively spliced isoforms, with one form particularly connected to obesity-related conditions. Located on human chromosome 16, Fatso′s gene resides in a region containing over 900 genes associated with various genetic disorders, including giant axonal neuropathy and Rubinstein-Taybi syndrome, underscoring Fatso′s significance in metabolic health and genetic disease.

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Fatso Antibody (C-3) References:

1. Cloning of Fatso (Fto), a novel gene deleted by the Fused toes (Ft) mouse mutation.  |  Peters, T., et al. 1999. Mamm Genome. 10: 983-6. PMID: 10501967
2. A common variant in the FTO gene is associated with body mass index and predisposes to childhood and adult obesity.  |  Frayling, TM., et al. 2007. Science. 316: 889-94. PMID: 17434869
3. Variation in FTO contributes to childhood obesity and severe adult obesity.  |  Dina, C., et al. 2007. Nat Genet. 39: 724-6. PMID: 17496892
4. Analysis of the obesity gene FTO in 14,803 type 1 diabetes cases and controls.  |  Field, SF., et al. 2007. Diabetologia. 50: 2218-20. PMID: 17657473
5. Genome-wide association scan shows genetic variants in the FTO gene are associated with obesity-related traits.  |  Scuteri, A., et al. 2007. PLoS Genet. 3: e115. PMID: 17658951
6. Genome-wide association studies provide new insights into type 2 diabetes aetiology.  |  Frayling, TM. 2007. Nat Rev Genet. 8: 657-62. PMID: 17703236
7. Common variants of the novel type 2 diabetes genes CDKAL1 and HHEX/IDE are associated with decreased pancreatic beta-cell function.  |  Pascoe, L., et al. 2007. Diabetes. 56: 3101-4. PMID: 17804762
8. The obesity-associated FTO gene encodes a 2-oxoglutarate-dependent nucleic acid demethylase.  |  Gerken, T., et al. 2007. Science. 318: 1469-72. PMID: 17991826
9. Fat mass and obesity-associated protein regulates RNA methylation associated with depression-like behavior in mice.  |  Liu, S., et al. 2021. Nat Commun. 12: 6937. PMID: 34836959
10. Fat mass and obesity-associated protein promotes liver steatosis by targeting PPARα.  |  Wei, X., et al. 2022. Lipids Health Dis. 21: 29. PMID: 35282837
11. Fat mass and obesity-associated protein regulates arecoline-exposed oral cancer immune response through programmed cell death-ligand 1.  |  Li, X., et al. 2022. Cancer Sci. 113: 2962-2973. PMID: 35289035
12. Fat mass and obesity-associated protein alleviates cerebral ischemia/reperfusion injury by inhibiting ferroptosis via miR-320-3p/SLC7A11 axis.  |  Peng, Q., et al. 2024. Biochem Pharmacol. 230: 116603. PMID: 39486461

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Fatso Antibody (C-3) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-Fatso Antibody (C-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Fatso Antibody (C-3) Specifications

Alternate Product Name: Fatso

Product Clone Name: C-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 252-505

Epitope Target: Fatso

Epitope Species: human

Product Reactivity/Detection: Fatso

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for Fatso Antibody (C-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin