FAT10 Antibody (G-5): sc-133199

FAT10 Antibody (G-5) is a mouse monoclonal IgG1 antibody that detects FAT10 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). FAT10, also known as Ubiquitin D or Diubiquitin, is a 165 amino acid protein encoded within the major histocompatibility complex (MHC) and features two domains that exhibit significant homology with ubiquitin. Each of these domains contains two cysteine residues and a free C-terminal diglycine motif, which is essential for the formation of FAT10 conjugates. FAT10 is notably inducible by interferon-γ and tumor necrosis factor alpha (TNFα), highlighting FAT10′s role in immune responses. FAT10 is crucial for various cellular processes, including antigen presentation, cytokine response, apoptosis, and mitosis, and FAT10 interacts with MAD2, a key component of the spindle checkpoint, thereby influencing cell cycle regulation. Expression of FAT10 mRNA is primarily observed in certain dendritic cells and lymphoblastoid lines, particularly following interferon-γ induction. Furthermore, the human FAT10 gene, designated UBD, is located on chromosome 6p21.3 and has been found to be overexpressed in tumors of various epithelial cancers, making FAT10 a significant marker for cancer research and potential therapeutic targeting. Anti-FAT10 antibody (G-5) is an invaluable tool for researchers investigating the multifaceted roles of FAT10 in cellular processes and disease mechanisms.

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FAT10 Antibody (G-5) References:

1. The ubiquitin-like modifier FAT10 in antigen processing and antimicrobial defense.  |  Basler, M., et al. 2015. Mol Immunol. 68: 129-32. PMID: 25983082
2. The ubiquitin-like modifier FAT10 in cancer development.  |  Aichem, A. and Groettrup, M. 2016. Int J Biochem Cell Biol. 79: 451-461. PMID: 27393295
3. Human Leukocyte Antigen F Locus Adjacent Transcript 10 Overexpression Disturbs WISP1 Protein and mRNA Expression to Promote Hepatocellular Carcinoma Progression.  |  Yan, J., et al. 2018. Hepatology. 68: 2268-2284. PMID: 29790184
4. Regulation of Interferon Induction by the Ubiquitin-Like Modifier FAT10.  |  Mah, MM., et al. 2020. Biomolecules. 10: PMID: 32586037
5. The ubiquitin-like modifier FAT10 - much more than a proteasome-targeting signal.  |  Aichem, A. and Groettrup, M. 2020. J Cell Sci. 133: PMID: 32719056
6. Ubiquitin-like protein FAT10: A potential cardioprotective factor and novel therapeutic target in cancer.  |  Zhang, K., et al. 2020. Clin Chim Acta. 510: 802-811. PMID: 32946796
7. NUB1 and FAT10 Proteins as Potential Novel Biomarkers in Cancer: A Translational Perspective.  |  Arshad, M., et al. 2021. Cells. 10: PMID: 34571823
8. The ubiquitin-like modifier FAT10 is induced in MASLD and impairs the lipid-regulatory activity of PPARα.  |  Clavreul, L., et al. 2024. Metabolism. 151: 155720. PMID: 37926201
9. Cytokines-activated nuclear IKKα-FAT10 pathway induces breast cancer tamoxifen-resistance.  |  Chen, X., et al. 2024. Sci China Life Sci. 67: 1413-1426. PMID: 38565741
10. FAT10 induces immune suppression by upregulating PD-L1 expression in hepatocellular carcinoma.  |  Wang, Q., et al. 2024. Apoptosis. 29: 1529-1545. PMID: 38824477

The preferred FAT10 antibody is FAT10 Antibody (A-8): sc-393630.