FAT10 Antibody (A-8): sc-393630

FAT10 Antibody (A-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects FAT10 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-FAT10 antibody (A-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FAT10, also known as Ubiquitin D or Diubiquitin, is a 165 amino acid protein encoded within the major histocompatibility complex (MHC) and features two domains that exhibit significant homology with ubiquitin. Each domain contains two cysteine residues and a free C-terminal diglycine motif, which is essential for the formation of FAT10 conjugates. FAT10 is notably inducible by interferon-γ and tumor necrosis factor alpha (TNFα), highlighting its role in immune responses. FAT10 is crucial for various cellular processes, including antigen presentation, cytokine response, apoptosis, and mitosis, and interacts with MAD2, a key component of the spindle checkpoint, thereby influencing cell cycle regulation. Additionally, FAT10 may play a role in regulating cell growth during the activation and development of dendritic cells and B cells. FAT10 mRNA expression is primarily observed in certain dendritic cells and lymphoblastoid lines, particularly following interferon-γ induction. The human FAT10 gene, designated UBD, is located on chromosome 6p21.3 and has been found to be overexpressed in tumors of various epithelial cancers, making FAT10 a significant target for research in cancer biology and immunology.

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FAT10 Antibody (A-8) References:

1. A MHC-encoded ubiquitin-like protein (FAT10) binds noncovalently to the spindle assembly checkpoint protein MAD2.  |  Liu, YC., et al. 1999. Proc Natl Acad Sci U S A. 96: 4313-8. PMID: 10200259
2. FAT10, a ubiquitin-independent signal for proteasomal degradation.  |  Hipp, MS., et al. 2005. Mol Cell Biol. 25: 3483-91. PMID: 15831455
3. FAT10 plays a role in the regulation of chromosomal stability.  |  Ren, J., et al. 2006. J Biol Chem. 281: 11413-21. PMID: 16495226
4. Role of ubiquitin-like protein FAT10 in epithelial apoptosis in renal disease.  |  Ross, MJ., et al. 2006. J Am Soc Nephrol. 17: 996-1004. PMID: 16495380
5. p53 negatively regulates the expression of FAT10, a gene upregulated in various cancers.  |  Zhang, DW., et al. 2006. Oncogene. 25: 2318-27. PMID: 16501612
6. FAT10, a gene up-regulated in various cancers, is cell-cycle regulated.  |  Lim, CB., et al. 2006. Cell Div. 1: 20. PMID: 16959044
7. UBA6 and Its Bispecific Pathways for Ubiquitin and FAT10.  |  Wang, F. and Zhao, B. 2019. Int J Mol Sci. 20: PMID: 31067743
8. FAT10 Silencing Prevents Liver Fibrosis through Regulating SIRT1 Expression in Hepatic Stellate Cells.  |  Zheng, W., et al. 2023. Int J Med Sci. 20: 557-565. PMID: 37057207
9. Ubiquitin-Like Protein FAT10 Promote Colorectal Cancer Progression by Affecting the Ubiquitination of Capn4.  |  Cheng, F., et al. 2023. Dig Dis Sci. 68: 3312-3323. PMID: 37310562
10. The ubiquitin-like protein FAT10 in hepatocellular carcinoma cells limits the efficacy of anti-VEGF therapy.  |  Qiu, Y., et al. 2024. J Adv Res. 59: 97-109. PMID: 37328057
11. Identification of seven new human MHC class I region genes around the HLA-F locus.  |  Fan, W., et al. 1996. Immunogenetics. 44: 97-103. PMID: 8662070
12. Identification and analysis of a novel member of the ubiquitin family expressed in dendritic cells and mature B cells.  |  Bates, EE., et al. 1997. Eur J Immunol. 27: 2471-7. PMID: 9368598

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FAT10 Antibody (A-8) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-FAT10 Antibody (A-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FAT10 Antibody (A-8) Specifications

Alternate Product Name: FAT10

Product Clone Name: A-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 112-139

Epitope Target: FAT10

Epitope Species: human

Product Reactivity/Detection: FAT10

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for FAT10 Antibody (A-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin