FADD Antibody (G-4): sc-271748

FADD Antibody (G-4) is a mouse monoclonal IgG2b kappa light chain antibody specifically designed to target FADD by recognizing its epitope spanning amino acids 28-209 at the C-terminus of human origin. FADD monoclonal antibody (G-4) effectively detects FADD from mouse, rat, and human species and is suitable for a wide range of applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Fas-Associated Death Domain (FADD), also known as GIG3, or MORT1, is a pivotal adaptor protein in the apoptosis signaling pathway, characterized by its conserved death domain structure, which is essential for interacting with death receptors such as FAS and TNFR1. The structural integrity of FADD′s death domain is crucial for recruiting and activating downstream caspases, thereby facilitating programmed cell death necessary for maintaining cellular homeostasis. Understanding FADD architecture provides valuable insights into its function in apoptosis and involvement in various diseases where cell death regulation is disrupted. FADD (G-4) antibody is available in both non-conjugated forms and multiple conjugated variants, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and a range of Alexa Fluor® conjugates, offering flexibility for diverse experimental needs. Anti-FADD antibody (G-4) is an essential tool for studying molecular mechanisms of apoptosis and intricate signaling pathways mediated by FADD, enabling detailed exploration of structural and functional dynamics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

FADD Antibody (G-4) References:

1. Nonapoptotic functions of FADD-binding death receptors and their signaling molecules.  |  Park, SM., et al. 2005. Curr Opin Cell Biol. 17: 610-6. PMID: 16226446
2. Inhibition of TNF receptor 1 internalization by adenovirus 14.7K as a novel immune escape mechanism.  |  Schneider-Brachert, W., et al. 2006. J Clin Invest. 116: 2901-13. PMID: 17024246
3. Apoptosis-associated gene expression in HIV-infected patients in response to successful antiretroviral therapy.  |  Balestrieri, E., et al. 2007. J Med Virol. 79: 111-7. PMID: 17177302
4. Focal adhesion kinase determines the fate of death or survival of cells in response to TNFalpha in the presence of actinomycin D.  |  Takahashi, R., et al. 2007. Biochim Biophys Acta. 1770: 518-26. PMID: 17197095
5. Decreased expressions of the TNF-alpha signaling adapters in peripheral blood mononuclear cells (PBMCs) are correlated with disease activity in patients with systemic lupus erythematosus.  |  Zhu, L., et al. 2007. Clin Rheumatol. 26: 1481-9. PMID: 17235653
6. FADD is a key regulator of lipid metabolism.  |  Zhuang, H., et al. 2016. EMBO Mol Med. 8: 895-918. PMID: 27357657
7. FADD Phosphorylation Modulates Blood Glucose Levels by Decreasing the Expression of Insulin-Degrading Enzyme.  |  Lin, Y., et al. 2020. Mol Cells. 43: 373-383. PMID: 32191993
8. The Fas death factor.  |  Nagata, S. and Golstein, P. 1995. Science. 267: 1449-56. PMID: 7533326
9. FAP-1: a protein tyrosine phosphatase that associates with Fas.  |  Sato, T., et al. 1995. Science. 268: 411-5. PMID: 7536343
10. The TNF receptor 1-associated protein TRADD signals cell death and NF-kappa B activation.  |  Hsu, H., et al. 1995. Cell. 81: 495-504. PMID: 7758105
11. The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death.  |  Smith, CA., et al. 1994. Cell. 76: 959-62. PMID: 8137429
12. Transducers of life and death: TNF receptor superfamily and associated proteins.  |  Baker, SJ. and Reddy, EP. 1996. Oncogene. 12: 1-9. PMID: 8552378

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FADD Antibody (G-4) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-FADD Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FADD Antibody (G-4) Specifications

Alternate Product Name: Fas associated death domain

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 28-209

Epitope Target: FADD

Epitope Species: human

Product Reactivity/Detection: FADD

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for FADD Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin