Factor IX Antibody (B-3): sc-377187
- Factor IX Antibody (B-3) is a mouse monoclonal IgG1 κ Factor IX antibody, cited in 5 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 301-339 within an internal region of Factor IX of human origin
- Factor IX Antibody (B-3) is recommended for detection of Factor IX of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Factor IX Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Factor IX Antibody (B-3) for WB applications. These reagents are now offered in bundles with Factor IX Antibody (B-3) (see ordering information below).
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Factor IX Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Factor IX protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Factor IX (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Factor IX protein plays a crucial role in the blood coagulation cascade, which is essential for hemostasis following tissue injury. Factor IX is a vitamin K-dependent serine protease synthesized in the liver as an inactive precursor, circulating in the bloodstream until activated. Upon activation, Factor IXa, which consists of a light chain and a heavy chain linked by disulfide bonds, catalyzes the conversion of Factor X to Xa, thereby amplifying the coagulation response. This process is vital for forming stable fibrin clots and promoting platelet aggregation, which are necessary to prevent excessive bleeding. Genetic mutations in Factor IX gene can lead to hemophilia B, a bleeding disorder that underscores Factor IX significance in maintaining normal hemostatic function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Factor IX Antibody (B-3) References:
- Adherence to prophylactic infusions of factor VIII or factor IX for haemophilia. | Thornburg, CD. and Pipe, SW. 2006. Haemophilia. 12: 198-9. PMID: 16476098
- An RNA aptamer that discriminates bovine factor IX from human factor IX. | Gopinath, SC., et al. 2006. J Biochem. 140: 667-76. PMID: 17030508
- Genetic determinants of immunogenicity to factor IX during the treatment of haemophilia B. | Saini, S., et al. 2015. Haemophilia. 21: 210-218. PMID: 25470321
- Active gamma-carboxylated human factor IX expressed using recombinant DNA techniques. | de la Salle, H., et al. Nature. 316: 268-70. PMID: 4040611
- Immune reactive factor IX in acquired factor IX deficiency. | Lechner, K. 1972. Thromb Diath Haemorrh. 27: 19-24. PMID: 5033668
- The isolation of prothrombin, Factor IX and Factor X from human Factor IX concentrates. | Modi, GJ., et al. 1984. Thromb Res. 36: 537-47. PMID: 6084874
- Assay of factor IX antigen using an enzyme immuno assay. | Parquet-Gernez, A., et al. 1984. Thromb Res. 35: 703-12. PMID: 6506026
- The activation of factor IX by the tissue factor pathway. | Rapaport, SI. 1981. Prog Clin Biol Res. 72: 57-76. PMID: 7335650
- Age-dependent effect on the level of factor IX. | Sweeney, JD. and Hoernig, LA. 1993. Am J Clin Pathol. 99: 687-8. PMID: 8322702
- Measurement of anti-factor IX IgG subclasses in haemophilia B patients who developed inhibitors with episodes of allergic reactions to factor IX concentrates. | Sawamoto, Y., et al. 1996. Thromb Res. 83: 279-86. PMID: 8870172
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Factor IX Antibody (B-3) | sc-377187 | 200 µg/ml | $316.00 | |||
Factor IX Antibody (B-3): m-IgG Fc BP-HRP Bundle | sc-529986 | 200 µg Ab; 10 µg BP | $354.00 | |||
Factor IX Antibody (B-3): m-IgGκ BP-HRP Bundle | sc-523226 | 200 µg Ab, 40 µg BP | $354.00 | |||
Factor IX Antibody (B-3) AC | sc-377187 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Factor IX Antibody (B-3) HRP | sc-377187 HRP | 200 µg/ml | $316.00 | |||
Factor IX Antibody (B-3) FITC | sc-377187 FITC | 200 µg/ml | $330.00 | |||
Factor IX Antibody (B-3) PE | sc-377187 PE | 200 µg/ml | $343.00 | |||
Factor IX Antibody (B-3) Alexa Fluor® 488 | sc-377187 AF488 | 200 µg/ml | $357.00 | |||
Factor IX Antibody (B-3) Alexa Fluor® 546 | sc-377187 AF546 | 200 µg/ml | $357.00 | |||
Factor IX Antibody (B-3) Alexa Fluor® 594 | sc-377187 AF594 | 200 µg/ml | $357.00 | |||
Factor IX Antibody (B-3) Alexa Fluor® 647 | sc-377187 AF647 | 200 µg/ml | $357.00 | |||
Factor IX Antibody (B-3) Alexa Fluor® 680 | sc-377187 AF680 | 200 µg/ml | $357.00 | |||
Factor IX Antibody (B-3) Alexa Fluor® 790 | sc-377187 AF790 | 200 µg/ml | $357.00 | |||
Factor IX (B-3) Neutralizing Peptide | sc-377187 P | 100 µg/0.5 ml | $68.00 |