Factor IX Antibody (AHIX-5041): sc-73457

Factor IX Antibody (AHIX-5041) is a mouse monoclonal IgG1 antibody that detects Factor IX in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Factor IX antibody (AHIX-5041) is available in a non-conjugated form, specifically designed for researchers studying the intricate mechanisms of hemostasis. Coagulation factor IX plays a crucial role in the blood coagulation cascade, which is vital for forming stable fibrin clots and facilitating platelet aggregation following tissue injury. This vitamin K-dependent serine protease is synthesized in the liver as an inactive precursor and is activated through proteolytic cleavage by factor XIa, resulting in factor IXa, which consists of a light chain and a heavy chain linked by disulfide bonds. The proper functioning of factor IX is essential, as genetic mutations at the Factor IX locus can lead to hemophilia B, a bleeding disorder that significantly impacts patient quality of life. Understanding the structure and function of factor IX is critical for developing therapeutic strategies and improving patient outcomes in conditions related to coagulation disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Factor IX Antibody (AHIX-5041) References:

1. Thrombin is a potent inducer of connective tissue growth factor production via proteolytic activation of protease-activated receptor-1.  |  Chambers, RC., et al. 2000. J Biol Chem. 275: 35584-91. PMID: 10952976
2. Exosite-mediated substrate recognition of factor IX by factor XIa. The factor XIa heavy chain is required for initial recognition of factor IX.  |  Ogawa, T., et al. 2005. J Biol Chem. 280: 23523-30. PMID: 15829482
3. The coagulation cascade: initiation, maintenance, and regulation.  |  Davie, EW., et al. 1991. Biochemistry. 30: 10363-70. PMID: 1931959
4. Expression and characterization of human factor IX and factor IX-factor X chimeras in mouse C127 cells.  |  Lin, SW., et al. 1990. J Biol Chem. 265: 144-50. PMID: 2294100
5. A sequential mechanism for exosite-mediated factor IX activation by factor XIa.  |  Geng, Y., et al. 2012. J Biol Chem. 287: 38200-9. PMID: 22961984
6. Basic mechanisms in blood coagulation.  |  Davie, EW. and Fujikawa, K. 1975. Annu Rev Biochem. 44: 799-829. PMID: 237463
7. Overestimation of N-glycoPEGylated factor IX activity in a one-stage factor IX clotting assay owing to silica-mediated premature conversion to activated factor IX.  |  Rosén, P., et al. 2016. J Thromb Haemost. 14: 1420-7. PMID: 27169618
8. Monitoring once-weekly recombinant factor IX prophylaxis in hemophilia B with thrombin generation assay and factor IX activity.  |  Nummi, V., et al. 2017. Int J Lab Hematol. 39: 359-368. PMID: 28406575
9. Severe factor VIII and factor IX deficiency in females.  |  Lusher, JM. and McMillan, CW. 1978. Am J Med. 65: 637-48. PMID: 309282
10. Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX.  |  Jaye, M., et al. 1983. Nucleic Acids Res. 11: 2325-35. PMID: 6687940
11. Isolation and characterization of a cDNA coding for human factor IX.  |  Kurachi, K. and Davie, EW. 1982. Proc Natl Acad Sci U S A. 79: 6461-4. PMID: 6959130
12. Human factor IX binds to specific sites on the collagenous domain of collagen IV.  |  Wolberg, AS., et al. 1997. J Biol Chem. 272: 16717-20. PMID: 9201971

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Factor IX Antibody (AHIX-5041) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Factor IX Antibody (AHIX-5041) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Factor IX Antibody (AHIX-5041) Specifications

Alternate Product Name: Factor IX

Product Clone Name: AHIX-5041

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: Factor IX

Epitope Species: human

Product Reactivity/Detection: Factor IX, Factor IXa and Factor IXa heavy chain

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide, 0.1% gelatin and < 1% glycerol

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Factor IX Antibody (AHIX-5041): N/A