EYA3 Antibody (G-9): sc-515626
- EYA3 Antibody (G-9) is a mouse monoclonal IgG1 κ EYA3 antibody provided at 200 µg/ml
- raised against amino acids 207-300 mapping within an internal region of EYA3 of human origin
- EYA3 Antibody (G-9) is recommended for detection of EYA3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-EYA3 Antibody (G-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EYA3 Antibody (G-9) for WB applications. These reagents are now offered in bundles with EYA3 Antibody (G-9) (see ordering information below).
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EYA3 Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects EYA3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EYA3 Antibody (G-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EYA3, or Eyes absent homolog 3, is a 573 amino acid protein that plays a crucial role in cellular processes, particularly in transcriptional regulation during organogenesis. EYA3 is primarily located in both the nucleus and cytoplasm, where nuclear presence is essential for gene expression regulation. EYA3 interacts with key proteins such as Six1, facilitating activation of genes involved in cellular proliferation and organ development. EYA3 undergoes post-translational modifications, including phosphorylation by ATM or ATR in response to DNA damage, which can influence activity and stability. EYA3 gene is located on chromosome 1, spanning approximately 260 million base pairs and constituting nearly 8% of the human genome, highlighting importance in genetic regulation and development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EYA3 Antibody (G-9) References:
- Cooperation of six and eya in activation of their target genes through nuclear translocation of Eya. | Ohto, H., et al. 1999. Mol Cell Biol. 19: 6815-24. PMID: 10490620
- Molecular interaction and synergistic activation of a promoter by Six, Eya, and Dach proteins mediated through CREB binding protein. | Ikeda, K., et al. 2002. Mol Cell Biol. 22: 6759-66. PMID: 12215533
- Eya protein phosphatase activity regulates Six1-Dach-Eya transcriptional effects in mammalian organogenesis. | Li, X., et al. 2003. Nature. 426: 247-54. PMID: 14628042
- ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. | Matsuoka, S., et al. 2007. Science. 316: 1160-6. PMID: 17525332
- A human homologue of the Drosophila eyes absent gene underlies branchio-oto-renal (BOR) syndrome and identifies a novel gene family. | Abdelhak, S., et al. 1997. Nat Genet. 15: 157-64. PMID: 9020840
- Cloning and characterization of two vertebrate homologs of the Drosophila eyes absent gene. | Zimmerman, JE., et al. 1997. Genome Res. 7: 128-41. PMID: 9049631
- Mouse Eya genes are expressed during limb tendon development and encode a transcriptional activation function. | Xu, PX., et al. 1997. Proc Natl Acad Sci U S A. 94: 11974-9. PMID: 9342347
- EYA4, a novel vertebrate gene related to Drosophila eyes absent. | Borsani, G., et al. 1999. Hum Mol Genet. 8: 11-23. PMID: 9887327
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EYA3 Antibody (G-9) | sc-515626 | 200 µg/ml | $316.00 | |||
EYA3 Antibody (G-9): m-IgG Fc BP-HRP Bundle | sc-531445 | 200 µg Ab; 10 µg BP | $354.00 | |||
EYA3 Antibody (G-9): m-IgGκ BP-HRP Bundle | sc-525135 | 200 µg Ab, 40 µg BP | $354.00 | |||
EYA3 Antibody (G-9): m-IgG1 BP-HRP Bundle | sc-544561 | 200 µg Ab; 20 µg BP | $354.00 | |||
EYA3 Antibody (G-9) AC | sc-515626 AC | 500 µg/ml, 25% agarose | $416.00 | |||
EYA3 Antibody (G-9) HRP | sc-515626 HRP | 200 µg/ml | $316.00 | |||
EYA3 Antibody (G-9) FITC | sc-515626 FITC | 200 µg/ml | $330.00 | |||
EYA3 Antibody (G-9) PE | sc-515626 PE | 200 µg/ml | $343.00 | |||
EYA3 Antibody (G-9) Alexa Fluor® 488 | sc-515626 AF488 | 200 µg/ml | $357.00 | |||
EYA3 Antibody (G-9) Alexa Fluor® 546 | sc-515626 AF546 | 200 µg/ml | $357.00 | |||
EYA3 Antibody (G-9) Alexa Fluor® 594 | sc-515626 AF594 | 200 µg/ml | $357.00 | |||
EYA3 Antibody (G-9) Alexa Fluor® 647 | sc-515626 AF647 | 200 µg/ml | $357.00 | |||
EYA3 Antibody (G-9) Alexa Fluor® 680 | sc-515626 AF680 | 200 µg/ml | $357.00 | |||
EYA3 Antibody (G-9) Alexa Fluor® 790 | sc-515626 AF790 | 200 µg/ml | $357.00 |