EXT2 Antibody (A-2): sc-514092
- EXT2 Antibody (A-2) is a mouse monoclonal IgG1 κ EXT2 antibody, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 402-537 mapping within an internal region of EXT2 of human origin
- EXT2 Antibody (A-2) is recommended for detection of EXT2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-EXT2 Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EXT2 Antibody (A-2) for WB applications. These reagents are now offered in bundles with EXT2 Antibody (A-2) (see ordering information below).
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EXT2 Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects EXT2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EXT2 Antibody (A-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EXT2 (A-2) antibody targets a protein that plays a crucial role in the biosynthesis of heparan sulfate, a glycosaminoglycan essential for various biological processes, including cell signaling, development, and tissue repair. Located primarily in the Golgi apparatus, EXT2 interacts with EXT1 to form heteromeric complexes that facilitate the polymerization of heparan sulfate. This interaction is vital because defects in EXT2 or EXT1 can lead to hereditary multiple exostoses (HME), an autosomal dominant disorder characterized by the formation of cartilage-capped bony protrusions on long bones. Proper functioning of EXT2 is critical for maintaining normal heparan sulfate biosynthesis, and transferase activities are necessary for the addition of D-glucuronyl and N-acetyl-D-glucosaminyl residues, which are fundamental for the structural integrity and biological activity of heparan sulfate. Anti-EXT2 antibody (A-2) serves as an important tool for studying the molecular mechanisms underlying HME and the broader implications of heparan sulfate in health and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EXT2 Antibody (A-2) References:
- The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparan sulfate. | McCormick, C., et al. 2000. Proc Natl Acad Sci U S A. 97: 668-73. PMID: 10639137
- Association of EXT1 and EXT2, hereditary multiple exostoses gene products, in Golgi apparatus. | Kobayashi, S., et al. 2000. Biochem Biophys Res Commun. 268: 860-7. PMID: 10679296
- Molecular basis of multiple exostoses: mutations in the EXT1 and EXT2 genes. | Wuyts, W. and Van Hul, W. 2000. Hum Mutat. 15: 220-7. PMID: 10679937
- The putative tumour suppressor EXT1 alters the expression of cell-surface heparan sulfate. | McCormick, C., et al. 1998. Nat Genet. 19: 158-61. PMID: 9620772
- The putative tumor suppressors EXT1 and EXT2 are glycosyltransferases required for the biosynthesis of heparan sulfate. | Lind, T., et al. 1998. J Biol Chem. 273: 26265-8. PMID: 9756849
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EXT2 Antibody (A-2) | sc-514092 | 200 µg/ml | $322.00 | |||
EXT2 Antibody (A-2): m-IgG Fc BP-HRP Bundle | sc-531028 | 200 µg Ab; 10 µg BP | $361.00 | |||
EXT2 Antibody (A-2): m-IgGκ BP-HRP Bundle | sc-524603 | 200 µg Ab, 40 µg BP | $361.00 | |||
EXT2 Antibody (A-2): m-IgG1 BP-HRP Bundle | sc-544364 | 200 µg Ab; 20 µg BP | $361.00 | |||
EXT2 Antibody (A-2) AC | sc-514092 AC | 500 µg/ml, 25% agarose | $424.00 | |||
EXT2 Antibody (A-2) HRP | sc-514092 HRP | 200 µg/ml | $322.00 | |||
EXT2 Antibody (A-2) FITC | sc-514092 FITC | 200 µg/ml | $336.00 | |||
EXT2 Antibody (A-2) PE | sc-514092 PE | 200 µg/ml | $349.00 | |||
EXT2 Antibody (A-2) Alexa Fluor® 488 | sc-514092 AF488 | 200 µg/ml | $364.00 | |||
EXT2 Antibody (A-2) Alexa Fluor® 546 | sc-514092 AF546 | 200 µg/ml | $364.00 | |||
EXT2 Antibody (A-2) Alexa Fluor® 594 | sc-514092 AF594 | 200 µg/ml | $364.00 | |||
EXT2 Antibody (A-2) Alexa Fluor® 647 | sc-514092 AF647 | 200 µg/ml | $364.00 | |||
EXT2 Antibody (A-2) Alexa Fluor® 680 | sc-514092 AF680 | 200 µg/ml | $364.00 | |||
EXT2 Antibody (A-2) Alexa Fluor® 790 | sc-514092 AF790 | 200 µg/ml | $364.00 |