ETAR Antibody (F-12): sc-515948
- ETAR Antibody (F-12) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 21-80 mapping within an N-terminal extracellular domain of ETAR of mouse origin
- Anti-ETAR Antibody (F-12) is recommended for detection of ETAR of mouse and rat origin by WB, IP, IF and ELISA
- Anti-ETAR Antibody (F-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ETAR Antibody (F-12) for WB applications. These reagents are now offered in bundles with ETAR Antibody (F-12) (see ordering information below).
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SEE ALSO...
ETAR Antibody (F-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Endothelin Receptor Type A (ETAR) in mouse and rat samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ETAR (F-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Endothelin receptor A (ETAR), also known as EDNRA, ET1 Receptor, ETA, EDN1, and ET-AR, is a crucial member of the guanine-binding regulatory protein-coupled receptor family, primarily responsible for mediating the effects of endothelins, particularly ET1, which ETAR binds with the highest affinity compared to the ETBR receptor. ETAR activation plays a significant role in various physiological processes, including vascular tone and blood pressure regulation, leading to increased cytoplasmic calcium concentration in cultured lens epithelial cells and inducing arachidonic acid accumulation. ETAR is characterized by seven hydrophobic transmembrane domains and is predominantly expressed in tissues such as aorta, lung, atrium, kidney, placenta, and prostate, with placental vascular smooth muscle cells (PVSMCs) being a unique expression site. ETAR localization and function understanding is vital, as dysregulation is implicated in various cardiovascular diseases, making ETAR a significant target for therapeutic intervention.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ETAR Antibody (F-12) References:
- The inhibitory influence of endothelin on active sodium-potassium transport in porcine lens. | Okafor, MC. and Delamere, NA. 2001. Invest Ophthalmol Vis Sci. 42: 1018-23. PMID: 11274080
- Cloning and characterization of cDNA encoding human A-type endothelin receptor. | Adachi, M., et al. 1991. Biochem Biophys Res Commun. 180: 1265-72. PMID: 1719979
- Cloning and functional expression of a vascular smooth muscle endothelin 1 receptor. | Lin, HY., et al. 1991. Proc Natl Acad Sci U S A. 88: 3185-9. PMID: 1849646
- Binding and functional properties of endothelin receptor subtypes in the human prostate. | Kobayashi, S., et al. 1994. Mol Pharmacol. 45: 306-11. PMID: 8114678
- Alternative RNA splicing of the human endothelin-A receptor generates multiple transcripts. | Miyamoto, Y., et al. 1996. Biochem J. 313 (Pt 3): 795-801. PMID: 8611157
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ETAR Antibody (F-12) | sc-515948 | 200 µg/ml | $316.00 | |||
ETAR Antibody (F-12): m-IgG Fc BP-HRP Bundle | sc-531565 | 200 µg Ab; 10 µg BP | $354.00 | |||
ETAR Antibody (F-12): m-IgGκ BP-HRP Bundle | sc-525293 | 200 µg Ab, 40 µg BP | $354.00 | |||
ETAR Antibody (F-12): m-IgG1 BP-HRP Bundle | sc-544625 | 200 µg Ab; 20 µg BP | $354.00 | |||
ETAR Antibody (F-12) AC | sc-515948 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ETAR Antibody (F-12) HRP | sc-515948 HRP | 200 µg/ml | $316.00 | |||
ETAR Antibody (F-12) FITC | sc-515948 FITC | 200 µg/ml | $330.00 | |||
ETAR Antibody (F-12) PE | sc-515948 PE | 200 µg/ml | $343.00 | |||
ETAR Antibody (F-12) Alexa Fluor® 488 | sc-515948 AF488 | 200 µg/ml | $357.00 | |||
ETAR Antibody (F-12) Alexa Fluor® 546 | sc-515948 AF546 | 200 µg/ml | $357.00 | |||
ETAR Antibody (F-12) Alexa Fluor® 594 | sc-515948 AF594 | 200 µg/ml | $357.00 | |||
ETAR Antibody (F-12) Alexa Fluor® 647 | sc-515948 AF647 | 200 µg/ml | $357.00 | |||
ETAR Antibody (F-12) Alexa Fluor® 680 | sc-515948 AF680 | 200 µg/ml | $357.00 | |||
ETAR Antibody (F-12) Alexa Fluor® 790 | sc-515948 AF790 | 200 µg/ml | $357.00 |