ERp72 Antibody (B-4): sc-390530

ERp72 Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects ERp72 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-ERp72 antibody (B-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ERp72 protein, also known as protein disulfide-isomerase A4, plays a crucial role in the endoplasmic reticulum by facilitating proper folding and assembly of proteins, particularly major histocompatibility complex (MHC) class I molecules. This process is vital for immune response, as MHC class I molecules present intracellularly derived peptides to cytotoxic T lymphocytes, enabling the immune system to recognize and eliminate infected or malignant cells. ERp72 interaction with other chaperones and proteins in the ER ensures that MHC class I molecules are correctly assembled before engaging with the transporter associated with antigen processing (TAP). By promoting correct formation of disulfide bonds and assisting in protein folding, ERp72 maintains cellular homeostasis and effective immune surveillance, making ERp72 a significant target for research in immunology and cell biology.

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ERp72 Antibody (B-4) References:

1. Mobilization of MHC class I molecules from late endosomes to the cell surface following activation of CD34-derived human Langerhans cells.  |  MacAry, PA., et al. 2001. Proc Natl Acad Sci U S A. 98: 3982-7. PMID: 11274420
2. Human deoxycytidine kinase. Sequence of cDNA clones and analysis of expression in cell lines with and without enzyme activity.  |  Huang, SH., et al. 1991. J Biol Chem. 266: 5353. PMID: 2002068
3. The disulfide isomerase ERp72 supports arterial thrombosis in mice.  |  Zhou, J., et al. 2017. Blood. 130: 817-828. PMID: 28576878
4. MicroRNA-378a-3p contributes to ovarian cancer progression through downregulating PDIA4.  |  Chanjiao, Y., et al. 2021. Immun Inflamm Dis. 9: 108-119. PMID: 33159506
5. PDIA4 promotes glioblastoma progression via the PI3K/AKT/m-TOR pathway.  |  Wang, M., et al. 2022. Biochem Biophys Res Commun. 597: 83-90. PMID: 35131603
6. PDIA4, a new endoplasmic reticulum stress protein, modulates insulin resistance and inflammation in skeletal muscle.  |  Lee, CH., et al. 2022. Front Endocrinol (Lausanne). 13: 1053882. PMID: 36619574
7. PDIA4 confers resistance to ferroptosis via induction of ATF4/SLC7A11 in renal cell carcinoma.  |  Kang, L., et al. 2023. Cell Death Dis. 14: 193. PMID: 36906674
8. LncRNA FAM225B Regulates PDIA4-Mediated Ovarian Cancer Cell Invasion and Migration via Modulating Transcription Factor DDX17.  |  Yao, C., et al. 2023. Breast J. 2023: 3970444. PMID: 37720188
9. Molecular cloning of the human glucose-regulated protein ERp57/GRP58, a thiol-dependent reductase. Identification of its secretory form and inducible expression by the oncogenic transformation.  |  Hirano, N., et al. 1995. Eur J Biochem. 234: 336-42. PMID: 8529662
10. Erp72 expression activated by transient cerebral ischemia or disturbance of neuronal endoplasmic reticulum calcium stores.  |  Paschen, W., et al. 1998. Metab Brain Dis. 13: 55-68. PMID: 9570640
11. The thiol oxidoreductase ERp57 is a component of the MHC class I peptide-loading complex.  |  Hughes, EA. and Cresswell, P. 1998. Curr Biol. 8: 709-12. PMID: 9637923
12. A role for the thiol-dependent reductase ERp57 in the assembly of MHC class I molecules.  |  Morrice, NA. and Powis, SJ. 1998. Curr Biol. 8: 713-6. PMID: 9637924

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ERp72 Antibody (B-4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-ERp72 Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ERp72 Antibody (B-4) Specifications

Alternate Product Name: ERp72

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 617-645

Epitope Target: ERp72

Epitope Species: human

Product Reactivity/Detection: ERp72

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine and avian

Additional Non-Mammalian Reactivity: avian

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for ERp72 Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin