ERp57 Antibody (4E69) recommended for detection of ERp57 of mouse, rat and human origin by WB, IP and IF

ERp57 Antibody (4E69): sc-71075

ERp57 Antibody (4E69) is rated 4.0 out of 5 by 3.
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Datasheets

    • ERp57 Antibody (4E69) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant human ERp57
    • recommended for detection of ERp57 of mouse, rat and human origin by WB, IP and IF; also reactive with additional species, including canine
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

What is the recommended fixation method for immunofluorescence staining with ERp57 (4E69): sc-71075 monoclonal antibody?

Asked by: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from WB WB, IHC and IHCP publishable data with human L-02, and liver - SCBT Publication Review
Date published: 2015-02-14
Rated 4 out of 5 by from Strong positive signal seen in WB in non Strong positive signal seen in WB in non-transfected 293T, human ERp57 transfected 293T and Daudi whole cell lysates. -SCBT QC
Date published: 2015-02-05
Rated 4 out of 5 by from Great immunofluorescence cytoplasmic staining Great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2013-06-12
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