ERGIC-53 Antibody (B-9): sc-271517
- ERGIC-53 Antibody (B-9) is a mouse monoclonal IgG2a κ, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 266-510 mapping at the C-terminus of ERGIC-53 of human origin
- recommended for detection of ERGIC-53 of mouse, rat and human origin by WB, IP, IF and ELISA
- See ERGIC-53 (F-3): sc-398777 for ERGIC-53 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ERGIC-53 Antibody (B-9) for WB applications. These reagents are now offered in bundles with ERGIC-53 Antibody (B-9) (see ordering information below).
ERGIC-53 Antibody (B-9) is a mouse monoclonal IgG2a antibody that detects ERGIC-53 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ERGIC-53, also known as LMAN1, plays a crucial role in the intracellular transport of glycoproteins, specifically those with high mannose-type glycans, from the endoplasmic reticulum to the ER-Golgi intermediate compartment. ERGIC-53 is essential for maintaining cellular homeostasis and ensuring that glycoproteins are correctly sorted and delivered to their destinations, which is vital for proper cell function and communication. Notably, mutations or null expression of ERGIC-53 can lead to severe consequences, including a rare autosomal recessive bleeding disorder characterized by a combined deficiency of coagulation factors V and VIII, highlighting the importance of ERGIC-53 in hemostasis. Anti-ERGIC-53 antibody (B-9) is an invaluable tool for researchers studying glycoprotein transport mechanisms and related pathologies, providing insights into the molecular underpinnings of various diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ERGIC-53 Antibody (B-9) References:
- The lectin ERGIC-53 is a cargo transport receptor for glycoproteins. | Appenzeller, C., et al. 1999. Nat Cell Biol. 1: 330-4. PMID: 10559958
- ERGIC-53 and traffic in the secretory pathway. | Hauri, HP., et al. 2000. J Cell Sci. 113 (Pt 4): 587-96. PMID: 10652252
- The sugar-binding ability of ERGIC-53 is enhanced by its interaction with MCFD2. | Kawasaki, N., et al. 2008. Blood. 111: 1972-9. PMID: 18056485
- Identification of ERGIC-53 as an intracellular transport receptor of alpha1-antitrypsin. | Nyfeler, B., et al. 2008. J Cell Biol. 180: 705-12. PMID: 18283111
- Subcellular localization of ERGIC-53 under endoplasmic reticulum stress condition. | Qin, SY., et al. 2012. Glycobiology. 22: 1709-20. PMID: 22821029
- Regulation of Mac-2BP secretion is mediated by its N-glycan binding to ERGIC-53. | Chen, Y., et al. 2013. Glycobiology. 23: 904-16. PMID: 23550150
- Hepatitis B Virus Exploits ERGIC-53 in Conjunction with COPII to Exit Cells. | Zeyen, L., et al. 2020. Cells. 9: PMID: 32806600
- Structure of full-length ERGIC-53 in complex with MCFD2 for cargo transport. | Watanabe, S., et al. 2024. Nat Commun. 15: 2404. PMID: 38493152
- Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway. | Itin, C., et al. 1995. J Cell Biol. 131: 57-67. PMID: 7559786
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERGIC-53 Antibody (B-9) | sc-271517 | 200 µg/ml | $316.00 | |||
ERGIC-53 Antibody (B-9): m-IgG Fc BP-HRP Bundle | sc-537651 | 200 µg Ab; 10 µg BP | $354.00 | |||
ERGIC-53 Antibody (B-9): m-IgGκ BP-HRP Bundle | sc-534985 | 200 µg Ab; 40 µg BP | $354.00 | |||
ERGIC-53 Antibody (B-9): m-IgG2a BP-HRP Bundle | sc-548077 | 200 µg Ab; 10 µg BP | $354.00 |