ERGIC-53 Antibody (C-6): sc-365158
- ERGIC-53 Antibody (C-6) is a mouse monoclonal IgG1 κ, cited in 27 publications, provided at 200 µg/ml
- raised against amino acids 266-510 mapping at the C-terminus of ERGIC-53 of human origin
- Anti-ERGIC-53 Antibody (C-6) is recommended for detection of ERGIC-53 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ERGIC-53 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for ERGIC-53 Antibody (C-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with ERGIC-53 Antibody (C-6) (see ordering information below).
ERGIC-53 Antibody (C-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects ERGIC-53 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-ERGIC-53 antibody (C-6) is offered in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. ERGIC-53, also known as LMAN1, plays a crucial role in intracellular transport of glycoproteins, specifically those with high mannose-type glycans, from the endoplasmic reticulum to the ER-Golgi intermediate compartment. ERGIC-53 functions as a mannose-specific lectin, recognizing sugar residues on glycoproteins and glycolipids, which is essential for sorting and recycling these molecules within the cell. Null expression of ERGIC-53 can lead to a rare autosomal recessive bleeding disorder characterized by a combined deficiency of coagulation factors V and VIII, highlighting ERGIC-53′s importance in maintaining hemostatic balance.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ERGIC-53 Antibody (C-6) References:
- Lectins and protein traffic early in the secretory pathway. | Hauri, HP., et al. 2002. Biochem Soc Symp. 73-82. PMID: 12655775
- LMAN1 is a molecular chaperone for the secretion of coagulation factor VIII. | Cunningham, MA., et al. 2003. J Thromb Haemost. 1: 2360-7. PMID: 14629470
- The binding of VIP36 and alpha-amylase in the secretory vesicles via high-mannose type glycans. | Hara-Kuge, S., et al. 2004. Glycobiology. 14: 739-44. PMID: 15070860
- Sugar-binding properties of VIP36, an intracellular animal lectin operating as a cargo receptor. | Kamiya, Y., et al. 2005. J Biol Chem. 280: 37178-82. PMID: 16129679
- Oligomerization and interacellular localization of the glycoprotein receptor ERGIC-53 is independent of disulfide bonds. | Neve, EP., et al. 2005. J Mol Biol. 354: 556-68. PMID: 16257008
- Cargo selectivity of the ERGIC-53/MCFD2 transport receptor complex. | Nyfeler, B., et al. 2006. Traffic. 7: 1473-81. PMID: 17010120
- ERp44 and ERGIC-53 synergize in coupling efficiency and fidelity of IgM polymerization and secretion. | Cortini, M. and Sitia, R. 2010. Traffic. 11: 651-9. PMID: 20102547
- LMAN1 (ERGIC-53) is a potential carrier protein for matrix metalloproteinase-9 glycoprotein secretion. | Duellman, T., et al. 2015. Biochem Biophys Res Commun. 464: 685-91. PMID: 26150355
- A dual role for COOH-terminal lysine residues in pre-Golgi retention and endocytosis of ERGIC-53. | Kappeler, F., et al. 1994. J Biol Chem. 269: 6279-81. PMID: 8119975
- ERGIC-53, a membrane protein of the ER-Golgi intermediate compartment, carries an ER retention motif. | Schindler, R., et al. 1993. Eur J Cell Biol. 61: 1-9. PMID: 8223692
- ERGIC-53 is a functional mannose-selective and calcium-dependent human homologue of leguminous lectins. | Itin, C., et al. 1996. Mol Biol Cell. 7: 483-93. PMID: 8868475
- The recycling of ERGIC-53 in the early secretory pathway. ERGIC-53 carries a cytosolic endoplasmic reticulum-exit determinant interacting with COPII. | Kappeler, F., et al. 1997. J Biol Chem. 272: 31801-8. PMID: 9395526
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ERGIC-53 Antibody (C-6) | sc-365158 | 200 µg/ml | $316.00 | |||
ERGIC-53 Antibody (C-6): m-IgG Fc BP-HRP Bundle | sc-525861 | 200 µg Ab; 10 µg BP | $354.00 | |||
ERGIC-53 Antibody (C-6) AC | sc-365158 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ERGIC-53 Antibody (C-6) HRP | sc-365158 HRP | 200 µg/ml | $316.00 | |||
ERGIC-53 Antibody (C-6) FITC | sc-365158 FITC | 200 µg/ml | $330.00 | |||
ERGIC-53 Antibody (C-6) PE | sc-365158 PE | 200 µg/ml | $343.00 | |||
ERGIC-53 Antibody (C-6) Alexa Fluor® 488 | sc-365158 AF488 | 200 µg/ml | $357.00 | |||
ERGIC-53 Antibody (C-6) Alexa Fluor® 546 | sc-365158 AF546 | 200 µg/ml | $357.00 | |||
ERGIC-53 Antibody (C-6) Alexa Fluor® 594 | sc-365158 AF594 | 200 µg/ml | $357.00 | |||
ERGIC-53 Antibody (C-6) Alexa Fluor® 647 | sc-365158 AF647 | 200 µg/ml | $357.00 | |||
ERGIC-53 Antibody (C-6) Alexa Fluor® 680 | sc-365158 AF680 | 200 µg/ml | $357.00 | |||
ERGIC-53 Antibody (C-6) Alexa Fluor® 790 | sc-365158 AF790 | 200 µg/ml | $357.00 |