eRF1 Antibody (F-1): sc-390427

eRF1 Antibody (F-1) is a mouse monoclonal IgG2b antibody that detects eRF1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). eRF1, or eukaryotic release factor 1, plays a crucial role in the termination of protein synthesis, a vital process for cellular function and homeostasis. eRF1 ensures that translation is accurately concluded by recognizing stop codons on the ribosome and facilitating the release of the newly synthesized polypeptide chain. eRF1′s importance is underscored by interaction with the GTP-binding protein eRF3, forming a quaternary complex that enhances the efficiency of termination. The highly conserved Asn-Ile-Lys-Ser (NIKS) tetrapeptide within eRF1 is essential for binding to the ribosome, highlighting the structural integrity required for function. The gene encoding human eRF1 is located on chromosome 5q31.2, and proper functioning is critical for maintaining the fidelity of protein synthesis, which is fundamental to cellular health and the prevention of diseases related to translation errors. Anti-eRF1 antibody (F-1) is an invaluable tool for researchers studying the mechanisms of translation termination and its implications in various biological processes.

The preferred eRF1 antibody is eRF1 Antibody (B-11): sc-365686.