eRF1 Antibody (B-11): sc-365686
- eRF1 Antibody (B-11) is a mouse monoclonal IgG2a κ eRF1 antibody, cited in 5 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 411-437 at the C-terminus of eRF1 of human origin
- eRF1 Antibody (B-11) is recommended for detection of eRF1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-eRF1 Antibody (B-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for eRF1 Antibody (B-11) for WB applications. These reagents are now offered in bundles with eRF1 Antibody (B-11) (see ordering information below).
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eRF1 Antibody (B-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects eRF1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). eRF1 (B-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eRF1 plays a crucial role in the termination of protein synthesis, a vital process that ensures the accurate completion of translation. eRF1 functions by recognizing stop codons in the ribosomal A site, where eRF1 promotes the hydrolysis of the last peptidyl-tRNA, effectively releasing the newly synthesized polypeptide chain. eRF1′s activity is significantly enhanced through interaction with the GTP-binding protein eRF3, forming a quaternary complex essential for function. This complex positions eRF1 correctly towards the stop codon and peptidyl-tRNA in the "GTP state" and facilitates the release of eRFs from the ribosome in the "GDP state." eRF1 contains a highly conserved Asn-Ile-Lys-Ser (NIKS) tetrapeptide, which is critical for interaction with the ribosome, underscoring the importance of structural integrity in the termination process. The gene encoding human eRF1 is located on chromosome 5q31.2, highlighting evolutionary conservation and significance across species.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
eRF1 Antibody (B-11) References:
- Evolution of eukaryotic translation elongation and termination factors: variations of evolutionary rate and genetic code deviations. | Moreira, D., et al. 2002. Mol Biol Evol. 19: 189-200. PMID: 11801747
- [A new method to measure the functional activity of class-1 translation termination factor eRF1]. | Mazur, AM., et al. 2002. Mol Biol (Mosk). 36: 129-35. PMID: 11862703
- Highly conserved NIKS tetrapeptide is functionally essential in eukaryotic translation termination factor eRF1. | Frolova, L., et al. 2002. RNA. 8: 129-36. PMID: 11911360
- Evaluation of ETF1/eRF1, mapping to 5q31, as a candidate myeloid tumor suppressor gene. | Dubourg, C., et al. 2002. Cancer Genet Cytogenet. 134: 33-7. PMID: 11996793
- Eukaryotic polypeptide chain release factor eRF3 is an eRF1- and ribosome-dependent guanosine triphosphatase. | Frolova, L., et al. 1996. RNA. 2: 334-41. PMID: 8634914
- Overexpression of human release factor 1 alone has an antisuppressor effect in human cells. | Le Goff, X., et al. 1997. Mol Cell Biol. 17: 3164-72. PMID: 9154815
- Functional expression of eukaryotic polypeptide chain release factors 1 and 3 by means of baculovirus/insect cells and complex formation between the factors. | Frolova, LY., et al. 1998. Eur J Biochem. 256: 36-44. PMID: 9746343
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eRF1 Antibody (B-11) | sc-365686 | 200 µg/ml | $322.00 | |||
eRF1 Antibody (B-11): m-IgG Fc BP-HRP Bundle | sc-529439 | 200 µg Ab; 10 µg BP | $361.00 | |||
eRF1 Antibody (B-11): m-IgGκ BP-HRP Bundle | sc-522329 | 200 µg Ab, 40 µg BP | $361.00 | |||
eRF1 Antibody (B-11): m-IgG2a BP-HRP Bundle | sc-547314 | 200 µg Ab; 10 µg BP | $361.00 | |||
eRF1 Antibody (B-11) AC | sc-365686 AC | 500 µg/ml, 25% agarose | $424.00 | |||
eRF1 Antibody (B-11) HRP | sc-365686 HRP | 200 µg/ml | $322.00 | |||
eRF1 Antibody (B-11) FITC | sc-365686 FITC | 200 µg/ml | $336.00 | |||
eRF1 Antibody (B-11) PE | sc-365686 PE | 200 µg/ml | $349.00 | |||
eRF1 Antibody (B-11) Alexa Fluor® 488 | sc-365686 AF488 | 200 µg/ml | $364.00 | |||
eRF1 Antibody (B-11) Alexa Fluor® 546 | sc-365686 AF546 | 200 µg/ml | $364.00 | |||
eRF1 Antibody (B-11) Alexa Fluor® 594 | sc-365686 AF594 | 200 µg/ml | $364.00 | |||
eRF1 Antibody (B-11) Alexa Fluor® 647 | sc-365686 AF647 | 200 µg/ml | $364.00 | |||
eRF1 Antibody (B-11) Alexa Fluor® 680 | sc-365686 AF680 | 200 µg/ml | $364.00 | |||
eRF1 Antibody (B-11) Alexa Fluor® 790 | sc-365686 AF790 | 200 µg/ml | $364.00 | |||
eRF1 (B-11) Neutralizing Peptide | sc-365686 P | 100 µg/0.5 ml | $69.00 |