ERCC1 Antibody (D-10): sc-17809

ERCC1 Antibody (D-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects ERCC1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-ERCC1 antibody (D-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ERCC1 plays a crucial role in the nucleotide excision repair pathway, which is essential for maintaining genomic stability by repairing DNA damage caused by environmental factors such as UV radiation. ERCC1 forms a heterodimer with XPF, a structure-specific endonuclease that is vital for the incision step during DNA repair. The ERCC1-XPF complex recognizes and cleaves damaged DNA, thereby facilitating the removal of bulky DNA adducts and preventing mutations that could lead to diseases such as xeroderma pigmentosum, a condition characterized by extreme sensitivity to sunlight and a predisposition to skin cancer. ERCC1′s interaction with various DNA repair proteins underscores its importance in cellular defense mechanisms against genotoxic stress, making ERCC1 a significant target for research in cancer biology and therapeutic interventions.

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ERCC1 Antibody (D-10) References:

1. Order of assembly of human DNA repair excision nuclease.  |  Wakasugi, M. and Sancar, A. 1999. J Biol Chem. 274: 18759-68. PMID: 10373492
2. Molecular characterization of the human excision repair gene ERCC-1: cDNA cloning and amino acid homology with the yeast DNA repair gene RAD10.  |  van Duin, M., et al. 1986. Cell. 44: 913-23. PMID: 2420469
3. ERCC1 as Predictor of Platinum Benefit in Non-Small-Cell Lung Cancer.  |  Postel-Vinay, S. and Soria, JC. 2017. J Clin Oncol. 35: 384-386. PMID: 27893323
4. Clinical Perspectives of ERCC1 in Bladder Cancer.  |  Koutsoukos, K., et al. 2020. Int J Mol Sci. 21: PMID: 33266377
5. Perspectives of ERCC1 in early-stage and advanced cervical cancer: From experiments to clinical applications.  |  Du, P., et al. 2022. Front Immunol. 13: 1065379. PMID: 36713431
6. Mammalian DNA nucleotide excision repair reconstituted with purified protein components.  |  Aboussekhra, A., et al. 1995. Cell. 80: 859-68. PMID: 7697716
7. Mutations in XPA that prevent association with ERCC1 are defective in nucleotide excision repair.  |  Li, L., et al. 1995. Mol Cell Biol. 15: 1993-8. PMID: 7891694
8. Co-correction of the ERCC1, ERCC4 and xeroderma pigmentosum group F DNA repair defects in vitro.  |  Biggerstaff, M., et al. 1993. EMBO J. 12: 3685-92. PMID: 8253090
9. Separation of protein factors that correct the defects in the seven complementation groups of xeroderma pigmentosum cells.  |  Tateishi, S., et al. 1995. J Biochem. 118: 819-24. PMID: 8576098
10. Roles of XPG and XPF/ERCC1 endonucleases in UV-induced immunostaining of PCNA in fibroblasts.  |  Miura, M., et al. 1996. Exp Cell Res. 226: 126-32. PMID: 8660947
11. Xeroderma pigmentosum group F caused by a defect in a structure-specific DNA repair endonuclease.  |  Sijbers, AM., et al. 1996. Cell. 86: 811-22. PMID: 8797827
12. ERCC1 mutations in UV-sensitive Chinese hamster ovary (CHO) cell lines.  |  Hayashi, T., et al. 1998. Mutat Res. 407: 269-76. PMID: 9653453

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ERCC1 Antibody (D-10) has 62 citations in a variety of scientific publications.

Primary antibodies like Anti-ERCC1 Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ERCC1 Antibody (D-10) Specifications

Alternate Product Name: ERCC1

Product Clone Name: D-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-297

Epitope Target: ERCC1

Epitope Species: human

Product Reactivity/Detection: ERCC1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 62 citations in various scientific publications

Available Conjugates/Forms for ERCC1 Antibody (D-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent