ER beta Antibody (1531) recommended for detection of ERβ of mouse, rat and human origin by WB, IP and IF

ERβ Antibody (1531): sc-53494

ERβ Antibody (1531) is rated 4.2 out of 5 by 13.
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Datasheets

    • ERβ Antibody (1531) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against ERβ, corresponding to amino acids 256-505 of human origin
    • Anti-ER beta Antibody (1531) is recommended for detection of ERβ of mouse, rat and human origin by WB, IP and IF; non cross-reactive with ERα
    • Anti-ER beta Antibody (1531) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ERβ (1531): sc-53494.
Submit a review for this product and receive 15 CruzCredits

I am working with some porcine samples. Do you know if this antibody will cross-react?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 13
Date published: 2017-01-09
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Rated 1 out of 5 by from Unquantifiable in WB for rat WAT Some bands showed up very, very faintly, other band didn't show up at all. Western blot (1:100) in rat white adipose tissue.
Date published: 2018-09-12
Rated 5 out of 5 by from Worked great for cell and tissue lysates for WB! Tested on human aortic SMCs and aortic tissue lysates at 1:100 dilution.
Date published: 2018-02-28
Rated 5 out of 5 by from Ishikawa細胞ではきれいにでます。 Ishikawa細胞のタンパクを10μg applyしました。比較的きれいにバンドを出すことができました。
Date published: 2018-02-23
Rated 5 out of 5 by from very nice band tested on rat tissue samples. It gave a strong band at 1/1000 dilution.
Date published: 2017-10-16
Rated 4 out of 5 by from good in immunofluorecence 1:50 dilution of this antibody was used in immunofluorecence staining in PFA fixed rat brain sections. It produced pretty good results.
Date published: 2017-08-30
Rated 5 out of 5 by from Strong western ! Very nice western blot results; strong signal with low background
Date published: 2017-01-26
Rated 5 out of 5 by from Reliable and Specific Antibody Specific for ER beta and not ER alpha. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-11
Rated 5 out of 5 by from This antibody works in many different human This antibody works in many different human cell lines such as T47D, MDA-MB-231 and MCF-7 by Western blot -SCBT Publication Review
Date published: 2015-04-14
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