ErbB4/HER4 Antibody (C-7): sc-8050

ErbB-4 Antibody (C-7) is a mouse monoclonal IgG2a kappa light chain antibody raised against human ErbB-4, a sophisticated signaling protein belonging to the epidermal growth factor receptor family. ErbB-4 mouse monoclonal antibody (C-7) recognizes an epitope within the ErbB-4 immunogen sequence and demonstrates broad species cross-reactivity, detecting ErbB-4 in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA) applications. Anti-ErbB-4 monoclonal antibody (C-7) is available in non-conjugated form and multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. ErbB-4 undergoes a unique regulatory mechanism through proteolytic processing: when ErbB-4′s ligand heregulin binds, ErbB-4 experiences a cleavage event that releases the ectodomain, initiating crucial downstream signaling cascades. This sophisticated control mechanism proves essential for normal cellular functions and takes on particular significance in cancer biology, especially in breast carcinoma where ErbB-4 expression levels become notably elevated. ErbB-4 maintains precise localization at the cell membrane, where ErbB-4 can form either homodimers or heterodimers with other receptor family members. These dimeric complexes create intricate signaling networks that maintain cellular homeostasis and orchestrate responses to external growth signals. Through these diverse cellular functions, ErbB-4 (C-7) antibody serves as a valuable tool for investigating both normal cell signaling and cancer development mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ErbB4/HER4 Antibody (C-7) References:

1. Roles of ErbB-3 and ErbB-4 in the physiology and pathology of the mammary gland.  |  Carraway, KL., et al. 1997. J Mammary Gland Biol Neoplasia. 2: 187-98. PMID: 10882304
2. Heregulin-dependent trafficking and cleavage of ErbB-4.  |  Zhou, W. and Carpenter, G. 2000. J Biol Chem. 275: 34737-43. PMID: 10944525
3. The basic biology of HER2.  |  Rubin, I. and Yarden, Y. 2001. Ann Oncol. 12 Suppl 1: S3-8. PMID: 11521719
4. erbB-1 and erbB-4 receptors act in concert to facilitate female sexual development and mature reproductive function.  |  Prevot, V., et al. 2005. Endocrinology. 146: 1465-72. PMID: 15591145
5. ErbB-4 and TNF-alpha converting enzyme localization to membrane microdomains.  |  Thiel, KW. and Carpenter, G. 2006. Biochem Biophys Res Commun. 350: 629-33. PMID: 17027649
6. Targeting ErbB-1 and ErbB-4 in irradiated head and neck cancer: results of in vitro and in vivo studies.  |  Barnea, I., et al. 2013. Head Neck. 35: 399-407. PMID: 22367849
7. Localization of the human HER4/erbB-4 gene to chromosome 2.  |  Zimonjic, DB., et al. 1995. Oncogene. 10: 1235-7. PMID: 7700649
8. Ligand-specific activation of HER4/p180erbB4, a fourth member of the epidermal growth factor receptor family.  |  Plowman, GD., et al. 1993. Proc Natl Acad Sci U S A. 90: 1746-50. PMID: 8383326
9. Selective cleavage of the heregulin receptor ErbB-4 by protein kinase C activation.  |  Vecchi, M., et al. 1996. J Biol Chem. 271: 18989-95. PMID: 8702564
10. Tyrosine phosphorylation and proteolysis. Pervanadate-induced, metalloprotease-dependent cleavage of the ErbB-4 receptor and amphiregulin.  |  Vecchi, M., et al. 1998. J Biol Chem. 273: 20589-95. PMID: 9685416
11. Expression of the c-erbB-4/HER4 protein and mRNA in normal human fetal and adult tissues and in a survey of nine solid tumour types.  |  Srinivasan, R., et al. 1998. J Pathol. 185: 236-45. PMID: 9771476
12. ErbB-4 mRNA expression is decreased in non-metastatic pancreatic cancer.  |  Graber, HU., et al. 1999. Int J Cancer. 84: 24-7. PMID: 9988227

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ErbB4/HER4 Antibody (C-7) has 52 citations in a variety of scientific publications.

Primary antibodies like Anti-ErbB4/HER4 Antibody (C-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ErbB4/HER4 Antibody (C-7) Specifications

Alternate Product Name: ERBB4

Product Clone Name: C-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1291-1308

Epitope Target: ErbB-4

Epitope Species: human

Product Reactivity/Detection: ErbB-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 52 citations in various scientific publications

Available Conjugates/Forms for ErbB4/HER4 Antibody (C-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin