EphB2 Antibody (2D12C6): sc-130068

EphB2 Antibody (2D12C6) is a mouse monoclonal IgG2b kappa light chain antibody that detects EphB2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-EphB2 antibody (2D12C6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EphB2 is a member of the Eph receptor family, which represents the largest group of receptor protein tyrosine kinases identified to date, playing a crucial role in mediating cell-cell interactions during development and in the central nervous system. EphB2 is primarily located on the cell surface, where EphB2 participates in signaling pathways that regulate cell adhesion, migration, and differentiation. Such localization is vital for function in neuronal development and synaptic plasticity, as EphB2 interacts with ligands, the ephrins, facilitating communication between adjacent cells. EphB2 interaction with ephrin-B2 is essential for establishing boundaries in the developing nervous system, influencing the organization of neural circuits. Understanding EphB2′s role in these processes is important for elucidating mechanisms underlying various neurological conditions and developmental disorders.

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EphB2 Antibody (2D12C6) References:

1. Interaction between the EPHB2 receptor and EFNB1 ligand drives gastric cancer invasion and metastasis via the Wnt/β-catenin/FAK pathway.  |  Hu, Y., et al. 2024. Int J Biol Macromol. 258: 128848. PMID: 38114003
2. Ubiquitin ligase and signalling hub MYCBP2 is required for efficient EPHB2 tyrosine kinase receptor function.  |  Chang, C., et al. 2024. Elife. 12: PMID: 38289221
3. Overexpression of EphB2 in the basolateral amygdala is crucial for inducing visceral pain sensitization in rats subjected to water avoidance stress.  |  Duan, GB., et al. 2024. CNS Neurosci Ther. 30: e14611. PMID: 38353051
4. Comprehensive pan-cancer analysis reveals EPHB2 is a novel predictive biomarker for prognosis and immunotherapy response.  |  Xu, S., et al. 2024. BMC Cancer. 24: 1064. PMID: 39198775
5. Identification and cloning of ELF-1, a developmentally expressed ligand for the Mek4 and Sek receptor tyrosine kinases.  |  Cheng, HJ. and Flanagan, JG. 1994. Cell. 79: 157-68. PMID: 7522971
6. Molecular cloning of a ligand for the EPH-related receptor protein-tyrosine kinase Htk.  |  Bennett, BD., et al. 1995. Proc Natl Acad Sci U S A. 92: 1866-70. PMID: 7534404
7. Identification of full-length and truncated forms of Ehk-3, a novel member of the Eph receptor tyrosine kinase family.  |  Valenzuela, DM., et al. 1995. Oncogene. 10: 1573-80. PMID: 7731712
8. Identification of alternatively spliced mRNAs encoding variants of MDK1, a novel receptor tyrosine kinase expressed in the murine nervous system.  |  Ciossek, T., et al. 1995. Oncogene. 10: 97-108. PMID: 7824284
9. Ligands for the receptor tyrosine kinases hek and elk: isolation of cDNAs encoding a family of proteins.  |  Kozlosky, CJ., et al. 1995. Oncogene. 10: 299-306. PMID: 7838529
10. cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases.  |  Fox, GM., et al. 1995. Oncogene. 10: 897-905. PMID: 7898931
11. Molecular characterization of a family of ligands for eph-related tyrosine kinase receptors.  |  Beckmann, MP., et al. 1994. EMBO J. 13: 3757-62. PMID: 8070404
12. Juxtamembrane tyrosine residues couple the Eph family receptor EphB2/Nuk to specific SH2 domain proteins in neuronal cells.  |  Holland, SJ., et al. 1997. EMBO J. 16: 3877-88. PMID: 9233798

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EphB2 Antibody (2D12C6) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-EphB2 Antibody (2D12C6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EphB2 Antibody (2D12C6) Specifications

Alternate Product Name: EphB2

Product Clone Name: 2D12C6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 17-200

Epitope Target: EphB2

Epitope Species: human

Product Reactivity/Detection: EphB2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for EphB2 Antibody (2D12C6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin