EPAS-1/HIF-2 alpha Antibody (190b): sc-13596

EPAS-1 Antibody (190b) is a mouse monoclonal IgG1 kappa light chain antibody that detects EPAS-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). EPAS-1 (190b) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. EPAS-1 protein, also known as HIF-2α, plays a crucial role in cellular responses to hypoxia, which is a condition characterized by low oxygen levels. EPAS-1 is primarily located in the nucleus, where EPAS-1 functions as a transcription factor that regulates the expression of genes involved in critical processes such as angiogenesis, erythropoiesis, and glucose metabolism. EPAS-1′s ability to form heterodimers with other hypoxia-inducible factors, such as HIF-1α and HIF-3α, enhances regulatory capacity, allowing cells to adapt to fluctuating oxygen levels effectively. This adaptability is vital for maintaining cellular homeostasis and supporting survival in various physiological and pathological conditions, including cancer and ischemic diseases. EPAS-1 monoclonal antibody (190b) enables researchers to gain valuable insights into the mechanisms of hypoxia and its impact on cellular function and disease progression.

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EPAS-1/HIF-2 alpha Antibody (190b) References:

1. The transcription factor EPAS-1/hypoxia-inducible factor 2alpha plays an important role in vascular remodeling.  |  Peng, J., et al. 2000. Proc Natl Acad Sci U S A. 97: 8386-91. PMID: 10880563
2. Hypoxia: adapting to high altitude by mutating EPAS-1, the gene encoding HIF-2α.  |  van Patot, MC. and Gassmann, M. 2011. High Alt Med Biol. 12: 157-67. PMID: 21718164
3. EPAS-1 mediates SP-1-dependent FBI-1 expression and regulates tumor cell survival and proliferation.  |  Wang, X., et al. 2014. Int J Mol Sci. 15: 15689-99. PMID: 25192290
4. Cross-talk between EPAS-1/HIF-2α and PXR signaling pathway regulates multi-drug resistance of stomach cancer cell.  |  Zhao, J., et al. 2016. Int J Biochem Cell Biol. 72: 73-88. PMID: 26783937
5. EPAS 1, congenital heart disease, and high altitude: disclosures by genetics, bioinformatics, and experimental embryology.  |  Sergi, C. 2019. Biosci Rep. 39: PMID: 31015364
6. Expression and prognostic significance of EPAS-1 in renal clear cell carcinoma.  |  Chen, B., et al. 2021. Ann Ital Chir. 92: 671-675. PMID: 34807005
7. Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension.  |  Wang, GL., et al. 1995. Proc Natl Acad Sci U S A. 92: 5510-4. PMID: 7539918
8. Endothelial PAS domain protein 1 (EPAS1), a transcription factor selectively expressed in endothelial cells.  |  Tian, H., et al. 1997. Genes Dev. 11: 72-82. PMID: 9000051
9. Molecular characterization of the murine Hif-1 alpha locus.  |  Luo, G., et al. 1997. Gene Expr. 6: 287-99. PMID: 9368100
10. Selection and analysis of a mutant cell line defective in the hypoxia-inducible factor-1 alpha-subunit (HIF-1alpha). Characterization of hif-1alpha-dependent and -independent hypoxia-inducible gene expression.  |  Wood, SM., et al. 1998. J Biol Chem. 273: 8360-8. PMID: 9525945
11. Role of HIF-1alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis.  |  Carmeliet, P., et al. 1998. Nature. 394: 485-90. PMID: 9697772
12. Molecular characterization and chromosomal localization of a third alpha-class hypoxia inducible factor subunit, HIF3alpha.  |  Gu, YZ., et al. 1998. Gene Expr. 7: 205-13. PMID: 9840812

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EPAS-1/HIF-2 alpha Antibody (190b) has 61 citations in a variety of scientific publications.

Primary antibodies like Anti-EPAS-1/HIF-2 alpha Antibody (190b) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EPAS-1/HIF-2 alpha Antibody (190b) Specifications

Alternate Product Name: EPAS1

Product Clone Name: 190b

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: EPAS-1

Epitope Species: human

Product Reactivity/Detection: EPAS-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 61 citations in various scientific publications

Available Conjugates/Forms for EPAS-1/HIF-2 alpha Antibody (190b): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred EPAS-1/HIF-2 alpha antibody is EPAS-1/HIF-2 alpha Antibody (A-5): sc-46691.