Ep-CAM Antibody (C-10): sc-25308

Ep-CAM Antibody (C-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ep-CAM of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-Ep-CAM antibody (C-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The epithelial cell adhesion molecule, also known as tumor-associated calcium signal transducer 1 and MK-1, is a crucial monomeric membrane glycoprotein predominantly expressed in normal human epithelial tissues and various carcinomas. The human Ep-CAM gene encodes a 314 amino acid protein that exists in two forms, with the major form being significantly reduced upon treatment with the amino-glycosylation inhibitor tunicamycin. Ep-CAM plays a vital role in cell adhesion, which is essential for maintaining tissue integrity and facilitating cellular communication. Ep-CAM′s overexpression in numerous carcinomas makes this protein a promising target for both visualization and therapeutic intervention of human solid tumors. Ep-CAM features an extracellular domain that includes two epidermal growth factor-like repeats, followed by a cysteine-poor region, which is critical for adhesive properties, thereby underscoring Ep-CAM′s importance in cancer biology and potential as a biomarker for tumor progression.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ep-CAM Antibody (C-10) References:

1. Molecular identification of a human carcinoma-associated glycoprotein antigen recognized by mouse monoclonal antibody FU-MK-1.  |  Tomita, Y., et al. 2000. Jpn J Cancer Res. 91: 231-8. PMID: 10761711
2. Oligomeric state of the colon carcinoma-associated glycoprotein GA733-2 (Ep-CAM/EGP40) and its role in GA733-mediated homotypic cell-cell adhesion.  |  Trebak, M., et al. 2001. J Biol Chem. 276: 2299-309. PMID: 11058587
3. Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions.  |  Balzar, M., et al. 2001. Mol Cell Biol. 21: 2570-80. PMID: 11259604
4. A murine cDNA encodes a pan-epithelial glycoprotein that is also expressed on plasma cells.  |  Bergsagel, PL., et al. 1992. J Immunol. 148: 590-6. PMID: 1729376
5. Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium.  |  Farr, A., et al. 1991. J Histochem Cytochem. 39: 645-53. PMID: 2016514
6. Epithelial cell adhesion molecule-1 (ECAM1) is required in the maintenance of corneal epithelial barrier integrity.  |  Zhou, J., et al. 2016. Cell Biol Int. 40: 49-54. PMID: 26269209
7. Epithelial cell adhesion molecule fragments and signaling in primary human liver cells.  |  Gerlach, JC., et al. 2018. J Cell Physiol. 233: 4841-4851. PMID: 29150960
8. Regulation of epithelial migration by epithelial cell adhesion molecule requires its Claudin-7 interaction domain.  |  Barth, AIM., et al. 2018. PLoS One. 13: e0204957. PMID: 30304739
9. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody.  |  Björk, P., et al. 1993. J Biol Chem. 268: 24232-41. PMID: 7693697
10. The murine homolog of human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells.  |  Nelson, AJ., et al. 1996. Eur J Immunol. 26: 401-8. PMID: 8617310
11. Epithelial cell adhesion molecule (Ep-CAM) modulates cell-cell interactions mediated by classic cadherins.  |  Litvinov, SV., et al. 1997. J Cell Biol. 139: 1337-48. PMID: 9382878
12. Abnormal thymic expression of epithelial cell adhesion molecule (EP-CAM) in New Zealand Black (NZB) mice.  |  Taguchi, N., et al. 1999. J Autoimmun. 13: 393-404. PMID: 10585755

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ep-CAM Antibody (C-10) has 83 citations in a variety of scientific publications.

Primary antibodies like Anti-Ep-CAM Antibody (C-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ep-CAM Antibody (C-10) Specifications

Alternate Product Name: Ep-CAM

Product Clone Name: C-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 24-93

Epitope Target: Ep-CAM

Epitope Species: human

Product Reactivity/Detection: Ep-CAM

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 83 citations in various scientific publications

Available Conjugates/Forms for Ep-CAM Antibody (C-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin