Ep-CAM Antibody (B302): sc-59783
- Ep-CAM Antibody (B302) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
- raised against MCF-7 human breast cancer cells of human origin
- recommended for detection of Ep-CAM of mouse, rat and human origin by WB, IP, IF and IHC(P); may cross-react with most epithelial cells and carcinomas
- See Ep-CAM (C-10): sc-25308 for Ep-CAM antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ep-CAM Antibody (B302) for WB and IHC(P) applications. These reagents are now offered in bundles with Ep-CAM Antibody (B302) (see ordering information below).
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Ep-CAM Antibody (B302) is a mouse monoclonal IgG1 antibody that detects Ep-CAM in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Ep-CAM, also known as tumor-associated calcium signal transducer 1 and MK-1, is a monomeric membrane glycoprotein predominantly expressed in normal human epithelial tissues and various carcinomas, making anti-Ep-CAM antibody (B302) a significant marker for cancer research. The human Ep-CAM gene encodes a 314 amino acid protein that exists in two forms, with the major form being more prevalent and the minor form being reduced upon treatment with the amino-glycosylation inhibitor tunicamycin. Notably, Ep-CAM plays a crucial role in cell adhesion due to its extracellular domain, which contains two epidermal growth factor-like repeats followed by a cysteine-poor region; this structural feature is essential for adhesive properties and contributes to the maintenance of tissue integrity. Given Ep-CAM′s overexpression in numerous carcinomas, Ep-CAM monoclonal antibody (B302) serves as a valuable tool for visualizing human solid tumors and holds potential for therapeutic interventions in cancer biology and treatment strategies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ep-CAM Antibody (B302) References:
- Epithelial cell adhesion molecule (KSA) expression: pathobiology and its role as an independent predictor of survival in renal cell carcinoma. | Seligson, DB., et al. 2004. Clin Cancer Res. 10: 2659-69. PMID: 15102668
- Increased ectodomain shedding of lung epithelial cell adhesion molecule 1 as a cause of increased alveolar cell apoptosis in emphysema. | Mimae, T., et al. 2014. Thorax. 69: 223-31. PMID: 24092566
- Hepatic stellate cells promote upregulation of epithelial cell adhesion molecule and epithelial-mesenchymal transition in hepatic cancer cells. | Nagahara, T., et al. 2015. Oncol Rep. 34: 1169-77. PMID: 26165819
- Synthesis of theranostic epithelial cell adhesion molecule targeted mesoporous silica nanoparticle with gold gatekeeper for hepatocellular carcinoma. | Babaei, M., et al. 2017. Nanomedicine (Lond). 12: 1261-1279. PMID: 28520529
- Epithelial cell adhesion molecule and epithelial-mesenchymal transition are associated with vasculogenic mimicry, poor prognosis, and metastasis of triple negative breast cancer. | Wu, Q., et al. 2019. Int J Clin Exp Pathol. 12: 1678-1689. PMID: 31933986
- Vascular Endothelial Growth Factor Promotes Proliferation of Epithelial Cell Adhesion Molecule-Positive Cells in Nonalcoholic Steatohepatitis. | Siddiqui, H., et al. 2020. J Clin Exp Hepatol. 10: 275-283. PMID: 32655229
- Ep-CAM (MOC-31) expression in tooth germ and ameloblastoma. | Derderian, N., et al. 2022. Med Oral Patol Oral Cir Bucal. 27: e403-e409. PMID: 35975801
- Multicoloured flow cytometry to detect metastatic carcinoma in lymph node by epithelial cell adhesion molecule. | Pradhan, N., et al. 2023. Diagn Cytopathol. 51: 191-195. PMID: 36409514
- Chimeric antigen receptor-T cells targeting epithelial cell adhesion molecule antigens are effective in the treatment of colorectal cancer. | Zeng, S., et al. 2024. BMC Gastroenterol. 24: 249. PMID: 39107717
- Cytoplasmic tail regulates the intercellular adhesion function of the epithelial cell adhesion molecule. | Balzar, M., et al. 1998. Mol Cell Biol. 18: 4833-43. PMID: 9671492
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ep-CAM Antibody (B302) | sc-59783 | 200 µg/ml | $322.00 | |||
Ep-CAM Antibody (B302): m-IgG Fc BP-HRP Bundle | sc-537168 | 200 µg Ab; 10 µg BP | $361.00 | |||
Ep-CAM Antibody (B302): m-IgGκ BP-HRP Bundle | sc-534366 | 200 µg Ab; 40 µg BP | $361.00 | |||
Ep-CAM Antibody (B302): m-IgG1 BP-HRP Bundle | sc-545048 | 200 µg Ab; 20 µg BP | $361.00 |