ENOPH1 Antibody (H-10): sc-365155

ENOPH1 Antibody (H-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects ENOPH1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ENOPH1 (H-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ENOPH1, also known as enolase-phosphatase 1, E1, MASA, or MST145, is a crucial enzyme belonging to the MasA family of the halo-acid dehalogenase-like hydrolase superfamily. ENOPH1 primarily functions in the cytoplasm, where ENOPH1 plays a vital role in the methionine salvage pathway, a biochemical process essential for maintaining methionine levels in cells across various organisms. Cytoplasmic localization of ENOPH1 allows for efficient access to substrates involved in methionine metabolism, thereby influencing cellular growth and function. ENOPH1 exhibits both phosphatase and atypical enolase activities, catalyzing the conversion of 2,3-diketo-5-methylthio-1-phosphopentane to the aci-reductone metabolite 1,2-dihydroxy-3-keto-5-methylthiopentene, which is critical for methionine recycling. Alternative splicing of the ENOPH1 gene results in two isoforms, further diversifying ENOPH1′s functional roles in cellular metabolism.

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ENOPH1 Antibody (H-10) References:

1. Analogs of 1-phosphonooxy-2,2-dihydroxy-3-oxo-5-(methylthio)pentane, an acyclic intermediate in the methionine salvage pathway: a new preparation and characterization of activity with E1 enolase/phosphatase from Klebsiella oxytoca.  |  Zhang, Y., et al. 2004. Bioorg Med Chem. 12: 3847-55. PMID: 15210152
2. (1)H, (13)C and (15)N chemical shift assignments of an enolase-phosphatase, E1, from Klebsiella oxytoca.  |  Kostic, M. and Pochapsky, TC. 2004. J Biomol NMR. 30: 359-60. PMID: 15756463
3. Crystal structure of human E1 enzyme and its complex with a substrate analog reveals the mechanism of its phosphatase/enolase activity.  |  Wang, H., et al. 2005. J Mol Biol. 348: 917-26. PMID: 15843022
4. The immediate-early ethylene response gene OsARD1 encodes an acireductone dioxygenase involved in recycling of the ethylene precursor S-adenosylmethionine.  |  Sauter, M., et al. 2005. Plant J. 44: 718-29. PMID: 16297065
5. Purification, crystallization and preliminary X-ray diffraction analysis of human enolase-phosphatase E1.  |  Wang, H., et al. 2005. Acta Crystallogr Sect F Struct Biol Cryst Commun. 61: 521-3. PMID: 16511085
6. OsMTN encodes a 5'-methylthioadenosine nucleosidase that is up-regulated during submergence-induced ethylene synthesis in rice (Oryza sativa L.).  |  Rzewuski, G., et al. 2007. J Exp Bot. 58: 1505-14. PMID: 17339651
7. Characterization of metal binding in the active sites of acireductone dioxygenase isoforms from Klebsiella ATCC 8724.  |  Chai, SC., et al. 2008. Biochemistry. 47: 2428-38. PMID: 18237192

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ENOPH1 Antibody (H-10) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ENOPH1 Antibody (H-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ENOPH1 Antibody (H-10) Specifications

Alternate Product Name: ENOPH1

Product Clone Name: H-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-261

Epitope Target: ENOPH1

Epitope Species: human

Product Reactivity/Detection: ENOPH1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ENOPH1 Antibody (H-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin