eIF5 Antibody (E-10): sc-28309

eIF5 Antibody (E-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects eIF5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-eIF5 antibody (E-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF5 (E-10) monoclonal antibody recognizes eukaryotic translation initiation factor 5, which plays a crucial role in protein synthesis initiation by catalyzing GTP hydrolysis bound to the 40S ribosomal subunit, essential for 80S initiation complex assembly. This process ensures correct ribosome assembly for protein synthesis initiation. eIF5 (E-10) antibody targets a protein that interacts with other initiation factors, such as eIF-2, a heterotrimer composed of eIF-2α, eIF-2β, and eIF-2γ subunits. Formation of eIF-2-GTP-tRNA complex facilitates ribosomal subunit binding to mRNA, representing a critical step in initiation. Understanding eIF5 function and protein interactions helps elucidate translation regulation mechanisms and their role in cellular processes, including growth and differentiation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

eIF5 Antibody (E-10) References:

1. Mechanism and regulation of eukaryotic protein synthesis.  |  Merrick, WC. 1992. Microbiol Rev. 56: 291-315. PMID: 1620067
2. Translational control in mammalian cells.  |  Hershey, JW. 1991. Annu Rev Biochem. 60: 717-55. PMID: 1883206
3. Overexpression of eIF5 or its protein mimic 5MP perturbs eIF2 function and induces ATF4 translation through delayed re-initiation.  |  Kozel, C., et al. 2016. Nucleic Acids Res. 44: 8704-8713. PMID: 27325740
4. A Case-Matched Gender Comparison Transcriptomic Screen Identifies eIF4E and eIF5 as Potential Prognostic Markers in Male Breast Cancer.  |  Humphries, MP., et al. 2017. Clin Cancer Res. 23: 2575-2583. PMID: 27986751
5. Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2. Characterization of the protein and its messenger RNA.  |  Ernst, H., et al. 1987. J Biol Chem. 262: 1206-12. PMID: 2948954
6. Human oncoprotein 5MP suppresses general and repeat-associated non-AUG translation via eIF3 by a common mechanism.  |  Singh, CR., et al. 2021. Cell Rep. 36: 109376. PMID: 34260931
7. Roles of HDAC2, eIF5, and eIF6 in Lung Cancer Tumorigenesis.  |  Cai, SX., et al. 2021. Curr Med Sci. 41: 764-769. PMID: 34403101
8. BZW1 Facilitates Glycolysis and Promotes Tumor Growth in Pancreatic Ductal Adenocarcinoma Through Potentiating eIF2α Phosphorylation.  |  Li, Z., et al. 2022. Gastroenterology. 162: 1256-1271.e14. PMID: 34951995
9. Cyst stem cell lineage eIF5 non-autonomously prevents testicular germ cell tumor formation via eIF1A/eIF2γ-mediated pre-initiation complex.  |  Li, Z., et al. 2022. Stem Cell Res Ther. 13: 351. PMID: 35883200
10. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.  |  Kozak, M. 1983. Microbiol Rev. 47: 1-45. PMID: 6343825
11. Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function.  |  Pause, A., et al. 1994. Nature. 371: 762-7. PMID: 7935836
12. Decreasing the level of translation initiation factor 4E with antisense RNA causes reversal of ras-mediated transformation and tumorigenesis of cloned rat embryo fibroblasts.  |  Rinker-Schaeffer, CW., et al. 1993. Int J Cancer. 55: 841-7. PMID: 8244582

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-eIF5 Antibody (E-10) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-eIF5 Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

eIF5 Antibody (E-10) Specifications

Alternate Product Name: eIF5

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: eIF5

Epitope Species: human

Product Reactivity/Detection: eIF5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for eIF5 Antibody (E-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin