Date published: 2026-4-12

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eIF5 Antibody (A-3): sc-48419

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Datasheets
  • eIF5 Antibody (A-3) is a mouse monoclonal IgG2b κ, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 1-300 of eIF5 of human origin
  • recommended for detection of eIF5 of mouse, rat and human origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for eIF5 Antibody (A-3) for WB applications. These reagents are now offered in bundles with eIF5 Antibody (A-3) (see ordering information below).
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eIF5 Antibody (A-3) is a mouse monoclonal IgG2b antibody that detects eIF5 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). eIF5 plays a crucial role in the regulation of translation initiation, a vital process for cellular function and growth, as eIF5 catalyzes the hydrolysis of GTP bound to the 40S ribosomal subunit, facilitating the assembly of the 80S initiation complex necessary for protein synthesis. Proper functioning of eIF5 is essential because dysregulation in translation initiation can lead to various diseases, including cancer, where overexpression of initiation factors can contribute to uncontrolled cell proliferation. eIF5 interacts with other initiation factors, such as eIF-2 and eIF-4E, forming a complex that is critical for the recruitment of ribosomal subunits and translation initiation. anti-eIF5 antibody (A-3) serves as an invaluable tool for researchers studying translation regulation mechanisms and disease implications.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

eIF5 Antibody (A-3)

sc-48419
200 µg/ml
$322.00

eIF5 Antibody (A-3): m-IgG Fc BP-HRP Bundle

sc-536839
200 µg Ab; 10 µg BP
$361.00

eIF5 Antibody (A-3): m-IgGκ BP-HRP Bundle

sc-534006
200 µg Ab; 40 µg BP
$361.00

eIF5 Antibody (A-3): m-IgG2b BP-HRP Bundle

sc-549835
200 µg Ab; 10 µg BP
$361.00

What is the recommended starting dilution for Western blot for sc-48419: eIF5 (A-3) monoclonal antibody?

Asked by: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Great antibody for Western blotGreat antibody for Western blot! Positive data in A-431, BC3H1, NIH/3T3, 3611-RF and KNRK whole cell lysates. -SCBT QC
Date published: 2013-08-08
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eIF5 Antibody (A-3) is rated 5.0 out of 5 by 1.
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