eIF4G Antibody (A-10): sc-133155

eIF4G Antibody (A-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects eIF4G protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-eIF4G antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF4G plays a crucial role in the initiation of protein synthesis in eukaryotic cells, functioning as a key component of the eukaryotic initiation factor 4F (eIF4F) complex, which is essential for the recruitment of ribosomes to messenger RNA (mRNA). This complex is vital for the translation process, as eIF4G serves as a scaffold that links eIF4E, which binds to the mRNA cap, and eIF4A, an ATP-dependent RNA helicase that unwinds mRNA secondary structures. By facilitating these interactions, eIF4G ensures efficient translation initiation, which is critical for proper cellular function and response to environmental signals. The ability of eIF4G to interact with various initiation factors and mRNA structures underscores its importance in regulating gene expression and maintaining cellular homeostasis.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

eIF4G Antibody (A-10) References:

1. Conducting the initiation of protein synthesis: the role of eIF4G.  |  Prévôt, D., et al. 2003. Biol Cell. 95: 141-56. PMID: 12867079
2. Amino acid sequence of the human protein synthesis initiation factor eIF-4 gamma.  |  Yan, R., et al. 1992. J Biol Chem. 267: 23226-31. PMID: 1429670
3. Eukaryotic initiation factors-4E and -4F stimulate 5' cap-dependent as well as internal initiation of protein synthesis.  |  Scheper, GC., et al. 1992. J Biol Chem. 267: 7269-74. PMID: 1559971
4. RNA unwinding in translation: assembly of helicase complex intermediates comprising eukaryotic initiation factors eIF-4F and eIF-4B.  |  Jaramillo, M., et al. 1991. Mol Cell Biol. 11: 5992-7. PMID: 1719376
5. Bidirectional RNA helicase activity of eucaryotic translation initiation factors 4A and 4F.  |  Rozen, F., et al. 1990. Mol Cell Biol. 10: 1134-44. PMID: 2304461
6. Isolation and mapping of a gene for protein synthesis initiation factor 4A and its expression during differentiation of murine erythroleukemia cells.  |  Reddy, NS., et al. 1988. Gene. 70: 231-43. PMID: 3215517
7. Amino acid sequence of the mRNA cap-binding protein from human tissues.  |  Rychlik, W., et al. 1987. Proc Natl Acad Sci U S A. 84: 945-9. PMID: 3469651
8. Development and Application of the CRISPR-dcas13d-eIF4G Translational Regulatory System to Inhibit Ferroptosis in Calcium Oxalate Crystal-Induced Kidney Injury.  |  He, Z., et al. 2024. Adv Sci (Weinh). 11: e2309234. PMID: 38380498
9. hnRNP R promotes O-GlcNAcylation of eIF4G and facilitates axonal protein synthesis.  |  Zare, A., et al. 2024. Nat Commun. 15: 7430. PMID: 39198412
10. Mapping of functional domains in eukaryotic protein synthesis initiation factor 4G (eIF4G) with picornaviral proteases. Implications for cap-dependent and cap-independent translational initiation.  |  Lamphear, BJ., et al. 1995. J Biol Chem. 270: 21975-83. PMID: 7665619
11. Eukaryotic protein synthesis: an in vitro analysis.  |  Merrick, WC. 1994. Biochimie. 76: 822-30. PMID: 7880898
12. eIF4G: translation's mystery factor begins to yield its secrets.  |  Morley, SJ., et al. 1997. RNA. 3: 1085-104. PMID: 9326485

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-eIF4G Antibody (A-10) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-eIF4G Antibody (A-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

eIF4G Antibody (A-10) Specifications

Alternate Product Name: EIF4G1

Product Clone Name: A-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: eIF4G

Epitope Species: human

Product Reactivity/Detection: eIF4G

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for eIF4G Antibody (A-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin