eIF4E Antibody (P-2): sc-9976

eIF4E Antibody (P-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects eIF4E of mouse, rat, human, and porcine origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-eIF4E antibody (P-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF4E plays a crucial role in the initiation of protein synthesis in eukaryotic cells, as eIF4E binds to the 5′ cap of mRNA, facilitating the recruitment of the ribosome to the mRNA transcript. This interaction is vital for the proper translation of mRNA into proteins, which are essential for numerous cellular functions and processes. eIF4E is part of the eIF4F complex, which also includes eIF4A and eIF4G; together, these proteins work to unwind mRNA secondary structures, allowing for efficient ribosome binding. Regulation of eIF4E activity is critical, as dysregulation can lead to various diseases, including cancer, making eIF4E an important target for therapeutic intervention and research.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

eIF4E Antibody (P-2) References:

1. Amino acid sequence of the human protein synthesis initiation factor eIF-4 gamma.  |  Yan, R., et al. 1992. J Biol Chem. 267: 23226-31. PMID: 1429670
2. Eukaryotic initiation factors-4E and -4F stimulate 5' cap-dependent as well as internal initiation of protein synthesis.  |  Scheper, GC., et al. 1992. J Biol Chem. 267: 7269-74. PMID: 1559971
3. RNA unwinding in translation: assembly of helicase complex intermediates comprising eukaryotic initiation factors eIF-4F and eIF-4B.  |  Jaramillo, M., et al. 1991. Mol Cell Biol. 11: 5992-7. PMID: 1719376
4. Bidirectional RNA helicase activity of eucaryotic translation initiation factors 4A and 4F.  |  Rozen, F., et al. 1990. Mol Cell Biol. 10: 1134-44. PMID: 2304461
5. eIF4E Resistance: Natural Variation Should Guide Gene Editing.  |  Bastet, A., et al. 2017. Trends Plant Sci. 22: 411-419. PMID: 28258958
6. Isolation and mapping of a gene for protein synthesis initiation factor 4A and its expression during differentiation of murine erythroleukemia cells.  |  Reddy, NS., et al. 1988. Gene. 70: 231-43. PMID: 3215517
7. Amino acid sequence of the mRNA cap-binding protein from human tissues.  |  Rychlik, W., et al. 1987. Proc Natl Acad Sci U S A. 84: 945-9. PMID: 3469651
8. eIF4E-homologous protein (4EHP): a multifarious cap-binding protein.  |  Christie, M. and Igreja, C. 2023. FEBS J. 290: 266-285. PMID: 34758096
9. eIF4E integrates into stress response.  |  Wu, X. and Qian, SB. 2024. Mol Cell. 84: 2009-2010. PMID: 38848688
10. eIF4E-independent translation is largely eIF3d-dependent.  |  Roiuk, M., et al. 2024. Nat Commun. 15: 6692. PMID: 39107322
11. Mapping of functional domains in eukaryotic protein synthesis initiation factor 4G (eIF4G) with picornaviral proteases. Implications for cap-dependent and cap-independent translational initiation.  |  Lamphear, BJ., et al. 1995. J Biol Chem. 270: 21975-83. PMID: 7665619
12. Eukaryotic protein synthesis: an in vitro analysis.  |  Merrick, WC. 1994. Biochimie. 76: 822-30. PMID: 7880898

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-eIF4E Antibody (P-2) has 136 citations in a variety of scientific publications.

Primary antibodies like Anti-eIF4E Antibody (P-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

eIF4E Antibody (P-2) Specifications

Alternate Product Name: eIF4E

Product Clone Name: P-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: eIF4E

Epitope Species: human

Product Reactivity/Detection: eIF4E

Species Reactivity/Detection: mouse, rat, human, porcine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 136 citations in various scientific publications

Available Conjugates/Forms for eIF4E Antibody (P-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin