eIF4E Antibody (A-10): sc-271480
- eIF4E Antibody (A-10) is a mouse monoclonal IgG2b κ, cited in 37 publications, provided at 200 µg/ml
- raised against amino acids 1-217 representing full length eIF4E of human origin
- Anti-eIF4E Antibody (A-10) is recommended for detection of eIF4E of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-eIF4E Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP is the preferred secondary detection reagent for eIF4E Antibody (A-10) for WB and IHC(P) applications. This reagent is now offered in a bundle with eIF4E Antibody (A-10) (see ordering information below).
eIF4E Antibody (A-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects eIF4E protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-eIF4E antibody (A-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. eIF4E protein plays a crucial role in the initiation of protein synthesis in eukaryotic cells, primarily by binding to the 5′ cap of mRNA, which is essential for the recruitment of the ribosome to the mRNA. This interaction facilitates the formation of the eukaryotic initiation complex eIF4F, which consists of eIF4E, eIF4A, and eIF4G. The complex promotes ribosome binding and helps in unwinding mRNA secondary structures, thereby enhancing translation efficiency. eIF4G serves as a scaffold that links eIF4E and eIF4A to the eIF3 complex, further underscoring eIF4E′s importance in regulating protein synthesis and cellular function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
eIF4E Antibody (A-10) References:
- The emerging roles of translation factor eIF4E in the nucleus. | Strudwick, S. and Borden, KL. 2002. Differentiation. 70: 10-22. PMID: 11963652
- eIF4E--from translation to transformation. | Mamane, Y., et al. 2004. Oncogene. 23: 3172-9. PMID: 15094766
- eIF4E, the mRNA cap-binding protein: from basic discovery to translational research. | Sonenberg, N. 2008. Biochem Cell Biol. 86: 178-83. PMID: 18443631
- Structure and functions of the translation initiation factor eIF4E and its role in cancer development and treatment. | Piserà, A., et al. 2018. J Genet Genomics. 45: 13-24. PMID: 29396141
- Biochemical and Structural Insights into the Eukaryotic Translation Initiation Factor eIF4E. | Volpon, L., et al. 2019. Curr Protein Pept Sci. 20: 525-535. PMID: 30636602
- Monitoring eIF4E-Dependent Nuclear 3' End mRNA Cleavage. | Delaleau, M. 2021. Methods Mol Biol. 2209: 347-361. PMID: 33201480
- The versatile relationships between eIF4E and eIF4E-interacting proteins. | Hernández, G. 2022. Trends Genet. 38: 801-804. PMID: 35568601
- miR-34b-3p Inhibition of eIF4E Causes Post-stroke Depression in Adult Mice. | Ke, X., et al. 2023. Neurosci Bull. 39: 194-212. PMID: 35802246
- The eukaryotic translation initiation factor eIF4E reprograms alternative splicing. | Ghram, M., et al. 2023. EMBO J. 42: e110496. PMID: 36843541
- Phospho-eIF4E stimulation regulates coronavirus entry by selective expression of cell membrane-residential factors. | Zhang, X-Z., et al. 2024. J Virol. 98: e0194823. PMID: 38299843
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
eIF4E Antibody (A-10) | sc-271480 | 200 µg/ml | $322.00 | |||
eIF4E Antibody (A-10): m-IgG2b BP-HRP Bundle | sc-548702 | 200 µg Ab; 10 µg BP | $361.00 | |||
eIF4E Antibody (A-10) AC | sc-271480 AC | 500 µg/ml, 25% agarose | $424.00 | |||
eIF4E Antibody (A-10) HRP | sc-271480 HRP | 200 µg/ml | $322.00 | |||
eIF4E Antibody (A-10) FITC | sc-271480 FITC | 200 µg/ml | $336.00 | |||
eIF4E Antibody (A-10) PE | sc-271480 PE | 200 µg/ml | $349.00 | |||
eIF4E Antibody (A-10) Alexa Fluor® 488 | sc-271480 AF488 | 200 µg/ml | $364.00 | |||
eIF4E Antibody (A-10) Alexa Fluor® 546 | sc-271480 AF546 | 200 µg/ml | $364.00 | |||
eIF4E Antibody (A-10) Alexa Fluor® 594 | sc-271480 AF594 | 200 µg/ml | $364.00 | |||
eIF4E Antibody (A-10) Alexa Fluor® 647 | sc-271480 AF647 | 200 µg/ml | $364.00 | |||
eIF4E Antibody (A-10) Alexa Fluor® 680 | sc-271480 AF680 | 200 µg/ml | $364.00 | |||
eIF4E Antibody (A-10) Alexa Fluor® 790 | sc-271480 AF790 | 200 µg/ml | $364.00 |