eIF4B Antibody (A-2): sc-390912

eIF4B Antibody (A-2) is a mouse monoclonal IgG1 antibody that detects eIF4B in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). eIF4B, or eukaryotic translation initiation factor 4B, is a crucial 611 amino acid protein that plays a significant role in the regulation of protein synthesis by facilitating the binding of mRNA to ribosomes, a process essential for the translation of genetic information into functional proteins. eIF4B contains one RNA recognition motif and interacts with other members of the eIF4 family, such as eIF4A, enhancing the ATP-dependent unwinding activity necessary for the initiation of translation. eIF4B′s importance extends beyond its structural role; eIF4B is vital for the proper assembly of the translation initiation complex, which ensures that protein synthesis occurs efficiently and accurately. The gene encoding eIF4B is located on human chromosome 12, a region that harbors over 1,100 genes and constitutes approximately 4.5% of the human genome, highlighting eIF4B′s significance in cellular function and gene expression regulation. With its ability to facilitate critical interactions in the early stages of translation, eIF4B is a key player in maintaining cellular homeostasis and responding to various physiological stimuli.

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eIF4B Antibody (A-2) References:

1. eIF4B controls survival and proliferation and is regulated by proto-oncogenic signaling pathways.  |  Shahbazian, D., et al. 2010. Cell Cycle. 9: 4106-9. PMID: 20948310
2. eIF4B phosphorylation by pim kinases plays a critical role in cellular transformation by Abl oncogenes.  |  Yang, J., et al. 2013. Cancer Res. 73: 4898-908. PMID: 23749639
3. eIF4B, eIF4G and RNA regulate eIF4A activity in translation initiation by modulating the eIF4A conformational cycle.  |  Harms, U., et al. 2014. Nucleic Acids Res. 42: 7911-22. PMID: 24848014
4. Neuronal BC RNAs cooperate with eIF4B to mediate activity-dependent translational control.  |  Eom, T., et al. 2014. J Cell Biol. 207: 237-52. PMID: 25332164
5. eIF4B is a convergent target and critical effector of oncogenic Pim and PI3K/Akt/mTOR signaling pathways in Abl transformants.  |  Chen, K., et al. 2016. Oncotarget. 7: 10073-89. PMID: 26848623
6. eIF4B stimulates translation of long mRNAs with structured 5' UTRs and low closed-loop potential but weak dependence on eIF4G.  |  Sen, ND., et al. 2016. Proc Natl Acad Sci U S A. 113: 10464-72. PMID: 27601676
7. eIF4B stimulates eIF4A ATPase and unwinding activities by direct interaction through its 7-repeats region.  |  Andreou, AZ., et al. 2017. RNA Biol. 14: 113-123. PMID: 27858515
8. Casein Kinase 2 dependent phosphorylation of eIF4B regulates BACE1 expression in Alzheimer's disease.  |  Bettegazzi, B., et al. 2021. Cell Death Dis. 12: 769. PMID: 34349120
9. Backbone resonance assignments of the C-terminal region of human translation initiation factor eIF4B.  |  Mondal, S., et al. 2023. Biomol NMR Assign. 17: 199-203. PMID: 37368134
10. Elaiophylin targets EIF4B to suppress the growth of esophageal squamous cell carcinoma via the PI3K/AKT signaling pathway.  |  Qiu, H., et al. 2024. Cancer Lett. 611: 217401. PMID: 39694222

The preferred eIF4B antibody is eIF4B Antibody (D-4): sc-376062.