eIF3β/eIF3I Antibody (A-8): sc-374155

eIF3β Antibody (A-8) is a mouse monoclonal IgG1 antibody that detects eIF3β in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). eIF3β is a critical component of the eukaryotic initiation factor 3 complex, which plays a pivotal role in the regulation of protein synthesis by facilitating the assembly of the 40S ribosomal subunit. eIF3β is located in the cytoplasm, where eIF3β interacts with other initiation factors and mRNA to form the pre-initiation complex necessary for translation. Proper localization of eIF3β is essential for efficient protein synthesis, as eIF3β ensures that the ribosomal subunits are correctly assembled and that translation initiation occurs smoothly. Notably, dysregulation of eIF3β has been implicated in various cancers, highlighting eIF3β′s importance not only in normal cellular function but also in the pathology of tumorigenesis. With insights into the mechanisms of protein synthesis and potential role in cancer, anti-eIF3β antibody (A-8) serves as an invaluable tool for researchers studying these critical biological processes.

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eIF3β/eIF3I Antibody (A-8) References:

1. Mutational analysis of mammalian translation initiation factor 5 (eIF5): role of interaction between the beta subunit of eIF2 and eIF5 in eIF5 function in vitro and in vivo.  |  Das, S. and Maitra, U. 2000. Mol Cell Biol. 20: 3942-50. PMID: 10805737
2. AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation.  |  Lee, KH., et al. 2014. Nucleic Acids Res. 42: 3590-606. PMID: 24423872
3. Neuronal Protein 3.1 Deficiency Leads to Reduced Cutaneous Scar Collagen Deposition and Tensile Strength due to Impaired Transforming Growth Factor-β1 to -β3 Translation.  |  Cheng, T., et al. 2017. Am J Pathol. 187: 292-303. PMID: 27939132
4. Comparative analysis of phosphorylation of translational initiation and elongation factors by seven protein kinases.  |  Tuazon, PT., et al. 1989. J Biol Chem. 264: 2773-7. PMID: 2914929
5. Knockdown of eukaryotic translation initiation factor 3 subunit B inhibits cell proliferation and migration and promotes apoptosis by downregulating WNT signaling pathway in acute myeloid leukemia.  |  Feng, Y. and Wu, L. 2020. Int J Clin Exp Pathol. 13: 99-106. PMID: 32055278
6. EIF3B promotes cancer progression in pancreatic cancer.  |  Zhu, H., et al. 2021. Scand J Gastroenterol. 56: 281-288. PMID: 33459066
7. EIF3B stabilizes PTGS2 expression by counteracting MDM2-mediated ubiquitination to promote the development and progression of malignant melanoma.  |  Fang, P., et al. 2022. Cancer Sci. 113: 4181-4192. PMID: 36050601
8. Identifying Genetic Signatures Associated with Oncogene-Induced Replication Stress in Osteosarcoma and Screening for Potential Targeted Drugs.  |  Gao, M., et al. 2024. Biochem Genet. 62: 1690-1715. PMID: 37672187
9. EIF3B stabilizes PCNA by counteracting SYVN1-mediated ubiquitination to serve as a promotor in cholangiocarcinoma.  |  Huang, R., et al. 2024. Aging (Albany NY). 16: 7311-7330. PMID: 38687509
10. Removal of beta subunit of the eukaryotic polypeptide chain initiation factor 2 by limited proteolysis.  |  Mitsui, K., et al. 1981. Proc Natl Acad Sci U S A. 78: 4128-32. PMID: 7027253

The preferred eIF3β/eIF3I antibody is eIF3β/eIF3I Antibody (A-7): sc-374156.