eIF2α Antibody (D-3): sc-133132

eIF2α Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody engineered to detect the eukaryotic initiation factor 2 alpha (eIF2α) protein from human, mouse, and rat sources across a variety of applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). eIF2α monoclonal antibody (D-3) targets the full-length human eIF2α epitope spanning amino acids 1-315, ensuring robust and reliable binding. eIF2α (D-3) antibody recognizes a critical component of the eukaryotic initiation complex, which also includes eIF2β and eIF2γ, and plays a pivotal role in the initiation of protein synthesis by facilitating the binding of tRNA to the 40S ribosomal subunit in a GTP-dependent manner. eIF2α (D-3) monoclonal antibody identifies a protein essential for the regulation of translation initiation and serves as a key regulatory node in cellular stress responses. When eIF2α is phosphorylated by specific kinases, eIF2α becomes stabilized and inhibits the GDP/GTP exchange necessary for translation initiation, thereby slowing down protein synthesis. This regulatory mechanism is crucial for cells to manage stress conditions and maintain homeostasis, and dysregulation of eIF2α phosphorylation is implicated in various diseases, including cancer and neurodegenerative disorders. eIF2α (D-3) antibody is available in both non-conjugated form and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatility for diverse experimental requirements and facilitating advanced research into the intricate mechanisms of protein synthesis and cellular stress responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

eIF2α Antibody (D-3) References:

1. A selective inhibitor of eIF2alpha dephosphorylation protects cells from ER stress.  |  Boyce, M., et al. 2005. Science. 307: 935-9. PMID: 15705855
2. Pi release from eIF2, not GTP hydrolysis, is the step controlled by start-site selection during eukaryotic translation initiation.  |  Algire, MA., et al. 2005. Mol Cell. 20: 251-62. PMID: 16246727
3. Expression and purification of the subunits of human translational initiation factor 2 (eIF2): phosphorylation of eIF2 alpha and beta.  |  Suragani, RN., et al. 2006. Protein Expr Purif. 47: 225-33. PMID: 16289913
4. The phosphorylation state of eucaryotic initiation factor 2 alters translational efficiency of specific mRNAs.  |  Kaufman, RJ., et al. 1989. Mol Cell Biol. 9: 946-58. PMID: 2657393
5. Binding and release of eukaryotic initiation factor eIF-2 and GTP during protein synthesis initiation.  |  Trachsel, H. and Staehelin, T. 1978. Proc Natl Acad Sci U S A. 75: 204-8. PMID: 272635
6. Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2. Characterization of the protein and its messenger RNA.  |  Ernst, H., et al. 1987. J Biol Chem. 262: 1206-12. PMID: 2948954
7. Structure of the beta subunit of translational initiation factor eIF-2.  |  Pathak, VK., et al. 1988. Cell. 54: 633-9. PMID: 3044606
8. The activity of eukaryotic initiation factor eIF-2 in ternary complex formation with GTP and Met-tRNA.  |  Benne, R., et al. 1979. J Biol Chem. 254: 3201-5. PMID: 429345
9. Translation initiation factor eIF-2. Cloning and expression of the human cDNA encoding the gamma-subunit.  |  Gaspar, NJ., et al. 1994. J Biol Chem. 269: 3415-22. PMID: 8106381

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-eIF2α Antibody (D-3) has 148 citations in a variety of scientific publications.

Primary antibodies like Anti-eIF2α Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

eIF2α Antibody (D-3) Specifications

Alternate Product Name: EIF2A

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: eIF2α

Epitope Species: human

Product Reactivity/Detection: eIF2α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 148 citations in various scientific publications

Available Conjugates/Forms for eIF2α Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin