EGR1 Antibody (B-6): sc-515830

Egr-1 Antibody (B-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Egr-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Egr-1 antibody (B-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Egr-1, a member of the early growth response (Egr) family of transcription factors, plays a crucial role in cellular processes such as mitogenesis and differentiation by binding to specific DNA sequences, thereby regulating transcription of target genes. Egr-1 (B-6) monoclonal antibody targets proteins primarily located in the nucleus, where Egr-1 acts as a transcriptional activator in response to extracellular stimuli, highlighting Egr-1′s importance in cellular signaling pathways. Egr-1 is rapidly induced by various signals, including growth factors and stress, and undergoes complex regulatory mechanisms, including post-translational modifications such as phosphorylation and glycosylation, which can influence activity and stability. Understanding Egr-1 function and regulation is vital, as dysregulation has been implicated in various diseases, including cancer and cardiovascular disorders, making Egr-1 (B-6) antibody an essential tool for researchers studying these critical biological processes.

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EGR1 Antibody (B-6) References:

1. Egr-1 negatively regulates calsequestrin expression and calcium dynamics in ventricular cells.  |  Kasneci, A., et al. 2009. Cardiovasc Res. 81: 695-702. PMID: 19103607
2. Egr-1 upregulates Siva-1 expression and induces cardiac fibroblast apoptosis.  |  Zins, K., et al. 2014. Int J Mol Sci. 15: 1538-53. PMID: 24451137
3. Egr-1 mediates chronic hypoxia-induced renal interstitial fibrosis via the PKC/ERK pathway.  |  Sun, S., et al. 2014. Am J Nephrol. 39: 436-48. PMID: 24819335
4. Egr-1 regulates irradiation-induced autophagy through Atg4B to promote radioresistance in hepatocellular carcinoma cells.  |  Peng, WX., et al. 2017. Oncogenesis. 6: e292. PMID: 28134935
5. Egr-1 Maintains NSC Proliferation and Its Overexpression Counteracts Cell Cycle Exit Triggered by the Withdrawal of Epidermal Growth Factor.  |  Cera, AA., et al. 2018. Dev Neurosci. 40: 223-233. PMID: 29975945
6. Egr-1 transactivates WNT5A gene expression to inhibit glucose-induced β-cell proliferation.  |  Wu, X., et al. 2018. Biochim Biophys Acta Gene Regul Mech.. PMID: 30025875
7. Temporal changes in Egr-1 and c-fos expression in rat models of myocardial ischemia.  |  Zhai, LQ., et al. 2021. Ann Palliat Med. 10: 1411-1420. PMID: 33183026
8. Klotho prevents epithelial-mesenchymal transition through Egr-1 downregulation in diabetic kidney disease.  |  Li, Y., et al. 2021. BMJ Open Diabetes Res Care. 9: PMID: 34099438
9. JNK-dependent phosphorylation and nuclear translocation of EGR-1 promotes cardiomyocyte apoptosis.  |  Zhou, J., et al. 2022. Apoptosis. 27: 246-260. PMID: 35103892
10. Mice with double knockout of Egr-1 and RCAN1 exhibit reduced inflammation during Pseudomonas aeruginosa lung infection.  |  Pang, Z. and Lin, TJ. 2023. Immunobiology. 228: 152377. PMID: 36933529

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EGR1 Antibody (B-6) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-EGR1 Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EGR1 Antibody (B-6) Specifications

Alternate Product Name: Egr-1

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 524-543

Epitope Target: Egr-1

Epitope Species: human

Product Reactivity/Detection: Egr-1 p82

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for EGR1 Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred EGR1 antibody is EGR1 Antibody (S-25): sc-101033.